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comparative evaluation of positive tests to mycobacterium avium subsp. paratuberculosis in clinically healthy sheep and goats in south-west greece using molecular techniques, serology, and culture.mycobacterium avium subsp. paratuberculosis (map) is the cause of paratuberculosis, which affects mainly ruminants although there is a growing concern about its possible implication in crohn's disease in humans especially in connection with environmental spread and risks to the food chain. retail cheese may represent a significant source of human exposure to map and the aim of this study was to assess map status in clinically healthy sheep and goats in greece, comparing techniques routinely used ...200717084095
identification and sub-typing of mycobacterium avium subsp. paratuberculosis and mycobacterium avium subsp. avium by randomly amplified polymorphic dna.a commercially available kit consisting of twenty 10-mer random primers was evaluated to allow selection of a suitable primer that would permit identification and sub-typing of mycobacterium avium subsp. paratuberculosis and mycobacterium avium subsp. avium by randomly amplified polymorphic dna (rapd). a primer ope-20 (5'-aac-ggt-gac-c-3') was identified to be the most suitable primer when tested with four atcc reference strains of m. paratuberculosis and eight well characterized field strains e ...200111240105
alkyl hydroperoxide reductases c and d are major antigens constitutively expressed by mycobacterium avium subsp. paratuberculosis.antigens characteristic for mycobacterium avium subspecies paratuberculosis were identified by crossed immunoelectrophoresis (cie) and by absorbing out cross-reactive antigens by using a polyclonal and polyvalent mycobacterium avium subspecies avium antiserum. two antigens were present in m. avium subsp. paratuberculosis and not detected in mycobacterium avium subsp. avium. they were identified as antigens 17 and 20 in a cie reference system for m. avium subsp. paratuberculosis antigens. purifie ...200010639449
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