| mycobacterial proteome extraction: comparison of disruption methods. | mycobacterium avium subsp. paratuberculosis has long been recognized as the causative agent of johne's disease, a chronic inflammatory intestinal disease of sheep, cattle and other ruminants. mycobacterial cells are extremely hardy, and proteomic analyses require the use of harsh conditions to effect their disruption. we compared the effectiveness of bead beating and sonication as cell lysis methods for the extraction of the proteomes of mycobacterium avium subsp. avium and mycobacterium avium s ... | 2004 | 15048990 |
| comparative evaluation of positive tests to mycobacterium avium subsp. paratuberculosis in clinically healthy sheep and goats in south-west greece using molecular techniques, serology, and culture. | mycobacterium avium subsp. paratuberculosis (map) is the cause of paratuberculosis, which affects mainly ruminants although there is a growing concern about its possible implication in crohn's disease in humans especially in connection with environmental spread and risks to the food chain. retail cheese may represent a significant source of human exposure to map and the aim of this study was to assess map status in clinically healthy sheep and goats in greece, comparing techniques routinely used ... | 2007 | 17084095 |
| isolation of high-affinity single-chain antibodies against mycobacterium avium subsp. paratuberculosis surface proteins from sheep with johne's disease. | johne's disease, caused by infection with mycobacterium avium subsp. paratuberculosis, causes significant economic losses to the livestock farming industry. improved investigative and diagnostic tools-necessary to understand disease processes and to identify subclinical infection-are much sought after. here, we describe the production of single-chain antibodies with defined specificity for m. avium subsp. paratuberculosis surface proteins. single-chain antibodies (scfv) were generated from sheep ... | 2006 | 16960114 |
| identification and characterization of an immunogenic 22 kda exported protein of mycobacterium avium subspecies paratuberculosis. | an exported 22 kda putative lipoprotein was identified in an alkaline phosphatase gene fusion library of mycobacterium avium subsp. paratuberculosis and expressed in mycobacterium smegmatis. the full nucleic acid sequence of the gene encoding p22 was determined and the orf was cloned into a mycobacterial expression vector, enabling full-length p22 to be produced as a c-terminal polyhistidine-tagged protein in m. smegmatis. n-terminal sequencing of the recombinant protein confirmed cleavage of a ... | 2005 | 16192441 |
| identification and sub-typing of mycobacterium avium subsp. paratuberculosis and mycobacterium avium subsp. avium by randomly amplified polymorphic dna. | a commercially available kit consisting of twenty 10-mer random primers was evaluated to allow selection of a suitable primer that would permit identification and sub-typing of mycobacterium avium subsp. paratuberculosis and mycobacterium avium subsp. avium by randomly amplified polymorphic dna (rapd). a primer ope-20 (5'-aac-ggt-gac-c-3') was identified to be the most suitable primer when tested with four atcc reference strains of m. paratuberculosis and eight well characterized field strains e ... | 2001 | 11240105 |
| pcr-restriction endonuclease analysis for identification and strain typing of mycobacterium avium subsp. paratuberculosis and mycobacterium avium subsp. avium based on polymorphisms in is1311. | point mutations in the is 1311 sequences from sheep and cattle strains of mycobacterium avium subsp. paratuberculosis (m. paratuberculosis) and m. avium subsp. avium (m. avium) were targeted to develop a polymerase chain reaction (pcr) that would be useful in the diagnosis and control of johne's disease. candidate pcr tests were evaluated for sensitivity, specificity and ease of interpretation of the restriction endonuclease analysis (rea) products. one is 1311 pcr, amplifying a 608 base pair pr ... | 1999 | 10208802 |