| two new cyclophilins from fusarium sambucinum and aspergillus niger: resistance of cyclophilin/cyclosporin a complexes against proteolysis. | two new peptidyl-prolyl-cis/trans-isomerases were purified to homogeneity from fusarium sambucinum and aspergillus niger. they belong to the class of cyclosporin a binding proteins (cyclophilins) and have molecular masses of about 18 kda. as has been shown for other cyclophilins, the isomerase activity of the enzymes is inhibited by cyclosporin a in the nanomolar range. furthermore binding of cyclosporin a prevents proteolytic digestion of the cyclophilin/cyclosporin complexes by the endoproteas ... | 1992 | 1530635 |
| thermophilic and thermotolerant fungi of animals' hair. | nine thermophilic genera and 17 species in addition to one variety of aspergillus flavus, malbranchea pulchella and humicola grisea were collected from hair samples in riyadh, saudi arabia at 45 degrees c. fifty-one hair specimens of rabbit, sheep, camel and horse were examined for the presence of thermophilic fungi. the most frequent species were aspergillus fumigatus, aspergillus niger, thermoascus aurantiacus and malbranchea pulchella var. sulfurea. in low frequency, aspergillus flavus, asper ... | 1991 | 1805498 |
| the efficacy of low temperature plasma (ltp) sterilization, a new sterilization technique. | the efficacy of low temperature plasma (ltp) sterilization, a newly developed sterilization procedure was tested. following experiments were carried out: determination of the most resistant test organism, influence of 10% and 20% defibrinated sheep blood or varying salt concentrations on the efficacy of the sterilization process, influence of the carrier position in the sterilization chamber and in the sterilization pouches, influence of a loaded sterilization chamber, comparative efficacy of eo ... | 1993 | 8397686 |
| release of volatile branched-chain and other fatty acids from ruminant milk fats by various lipases. | bovine, ovine, and caprine milk fats were treated with pregastric lipases (kid goat, calf, and lamb), microbial lipases (candida cylindracea, c. cylindracea ay30, aspergillus niger apf12, rhizopus arrhizus, penicillium roqueforti r10, and mucor zavanicus map 10), porcine pancreatic lipase, or milk lipase. all three pregastric lipases preferentially hydrolyzed volatile branched-chain and short n-chain fatty acids from each milk fat. pregastric lipases also released a relatively low proportion of ... | 1993 | 8463483 |
| biodegradation of wool by trichophyton simii and aspergillus niger. | the amount of protein released into liquid culture medium and weight loss were taken as measures of wool degradation by trichophyton simii and aspergillus niger. protein released into the culture medium was observed to be greater after t. simii treatment than after a. niger treatment. sulphitolysis was shown by t. simii, whereas it was absent in the case of a. niger, ph was found to be alkaline with an increase in soluble protein in the culture medium in the case of both uniculture and dual cult ... | 1996 | 9145010 |
| stability and stabilization of potential feed additive enzymes in rumen fluid* | four commercial preparations of fibrolytic enzymes, from irpex lacteus, trichoderma viride, aspergillus niger, and a mixture designed to be similar to the i. lacteus extract, were incubated in vitro with digesta taken from the rumen of sheep receiving a grass hay/concentrate diet, and the survival of major enzyme activities was measured. some activities, including the beta-1,4-endoglucanase and xylanase from the extract derived from aspergillus niger, were stable for at least 6 h in rumen fluid. ... | 2000 | 10689074 |
| a discussion of anti-aspergillus niger glucose oxidase monoclonal antibody reactivity to red blood cells of several species. | we observed that mouse spleen cells from rosettes with autologous red blood cells (rbcs) and that rosette-formation was suppressed by anti-aspergillus niger glucose oxidase monoclonal antibody (mab). in the present study, we investigated whether rbcs of species besides mice have the structure recognized by anti-a. niger glucose oxidase mab by using rosette-formation and complement-mediated hemolysis. lysates of monkey and human rbcs did not suppress rosette-formation whereas autologous (mouse), ... | 2000 | 10911721 |
| an anthelmintic compound, nafuredin, shows selective inhibition of complex i in helminth mitochondria. | infections with parasitic helminths are important causes of morbidity and mortality worldwide. new drugs that are parasite specific and minimally toxic to the host are needed to counter these infections effectively. here we report the finding of a previously unidentified compound, nafuredin, from aspergillus niger. nafuredin inhibits nadh-fumarate reductase (complexes i + ii) activity, a unique anaerobic electron transport system in helminth mitochondria, at nm order. it competes for the quinone ... | 2001 | 11120889 |
| enhancement of citric acid production with ram horn hydrolysate by aspergillus niger. | the potential use of ram horn hydrolysate (rhh) as a supplement for improvement of citric acid production by aspergillus niger nrrl 330 was studied. for this purpose, first rhh was produced. ram horns were hydrolyzed by treating with acid (6 n-h2so4) and the rhh was obtained. with the addition of rhh to the fermentation medium with a final concentration of 4% (optimal concentration), citric acid value reached a maximum value (94 g/l), which is 52% higher than that of the control experiment. the ... | 2004 | 14643992 |
| ram horn peptone as a source of citric acid production by aspergillus niger, with a process. | the present study deals with the production of citric acid from a ram horn peptone (rhp) by aspergillus niger nrrl 330. a medium from rhp and a control medium (cm) were compared for citric acid production using a. niger in a batch culture. for this purpose, first, rhp was produced. ram horns were hydrolyzed by treatment with acids (6 n h(2)so(4), 6 n hcl) and neutralizing solutions. the amounts of protein, nitrogen, ash, some minerals, total sugars, total lipids and amino acids of the rhp were d ... | 2004 | 15248090 |
| effects of exogenous cellulase supplementation on microbial growth and ruminal fermentation of a high-forage diet in rusitec fermenters. | two incubation runs were carried out with a rusitec system to investigate the effects of 2 exogenous pure cellulases on ruminal microbial growth and fermentation of a 70:30 grass hay:concentrate (dm basis) substrate. the substrate was sprayed with buffer (control; ph = 6.5), a cellulase from trichoderma longibrachiatum (tri), a cellulase from aspergillus niger (asp), or a 1:1 mixture of both cellulases (mix) 24 h before being placed in the fermenters. enzymes were applied at a rate of 30 endoglu ... | 2007 | 17468414 |
| production of (r)-1-(4-bromo-phenyl)-ethanol by locally isolated aspergillus niger using ram horn peptone. | aspergillus niger ebk-9 was isolated from soil sample. this isolate was evaluated for production of (r)-1-(4-bromo-phenyl)-ethanol 2 from 1-(4-bromo-phenyl)-ethanone 1. in this work, the production of the 2 was achieved via fermenter. glucose, yeast extract and ram horn peptone as medium in fermenter for growth of a. niger was used. a. niger ebk-9 isolate was found to be an effective biocatalyst with excellent enantiomeric excess (>99%) and good conversion (100%) for the production of the 2 in b ... | 2008 | 17531472 |
| screening of aspergillus species for occurrence of lectins and their characterization. | ten species of aspergillus were screened for occurrence of lectins. each of the species was investigated for the occurrence of extracellular, surface-bound and intracellular lectin activities. as many as four species namely, aspergillus niger, aspergillus versicolor, aspergillus rugulosus and aspergillus nidulans, were found to possess intracellular lectin activities, while none of the species showed extracellular or surface-bound lectin activities. each of the lectin was characterized with resp ... | 2008 | 18383222 |
| putative aspergillus niger-induced oxalate nephrosis in sheep. | a sheep farmer provided a maize-based brewer's grain (mieliemaroek) and bales of eragrostis curvula hay to ewes and their lambs, kept on zero-grazing in pens. the 'mieliemaroek' was visibly mouldy. after 14 days in the feedlot, clinical signs, including generalised weakness, ataxia of the hind limbs, tremors and recumbency, were noticed. six ewes died within a period of 7 days. a post mortem examination was performed on 1 ewe. the carcass appeared to be cachectic with mild effusions into the bod ... | 2009 | 19653520 |
| a single amino acid change (y318f) in the l-arabitol dehydrogenase (lada) from aspergillus niger results in a significant increase in affinity for d-sorbitol. | l-arabitol dehydrogenase (lad) and xylitol dehydrogenase (xdh) are involved in the degradation of l-arabinose and d-xylose, which are among the most abundant monosaccharides on earth. previous data demonstrated that lad and xdh not only differ in the activity on their biological substrate, but also that only xdh has significant activity on d-sorbitol and may therefore be more closely related to d-sorbitol dehydrogenases (sdh). in this study we aimed to identify residues involved in the differenc ... | 2009 | 19674460 |
| utilization of ram horn peptone in the production of glucose oxidase by a local isolate aspergillus niger oc-3. | glucose oxidase (go) is an enzyme that is used in many fields. in this study, ram horn peptone (rhp) was utilized as the nitrogen source and compared with other nitrogen sources in the production of go by aspergillus niger. to obtain higher go activity, 14 a. niger strains were isolated from soil samples around erzurum, turkey. among these strains, the isolate that was named a. niger oc-3 achieved the highest go production. the production of go was carried out in 100 ml scaled batch culture. the ... | 2011 | 21229465 |
| the pattern of sensitisation to inhalant allergens in omani patients with asthma, allergic rhinitis and rhinoconjunctivitis. | identification of relevant allergens that are prevalent in each environment which may have diagnostic and therapeutic implications in allergic diseases. this study aimed to identify the pattern of sensitisation to inhalant allergens in omani patients with asthma, allergic rhinitis and rhinoconjunctivitis. | 2008 | 21748078 |
| mitochondrial fumarate reductase as a target of chemotherapy: from parasites to cancer cells. | recent research on respiratory chain of the parasitic helminth, ascaris suum has shown that the mitochondrial nadh-fumarate reductase system (fumarate respiration), which is composed of complex i (nadh-rhodoquinone reductase), rhodoquinone and complex ii (rhodoquinol-fumarate reductase) plays an important role in the anaerobic energy metabolism of adult parasites inhabiting hosts. the enzymes in these parasite-specific pathways are potential target for chemotherapy. we isolated a novel compound, ... | 2011 | 22226661 |