Publications

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expression of the nucleocapsid protein of dugbe virus and antigenic cross-reactions with other nairoviruses.the small (s) rna segment of dugbe (dug) virus (nairovirus, bunyaviridae) encodes a single protein, the nucleocapsid (n) protein, of m(r) 49.4 kda. cdna derived from the complete coding region for the n protein was cloned into autographa californica nuclear polyhedrosis virus (acnpv) under control of the polyhedrin promoter and used to infect spodoptera frugiperda insect cells. western blotting analysis using monoclonal antibodies demonstrated the production of dug n protein in the infected cell ...19921529645
repeat sequences from complex ds dna viruses can be used as minisatellite probes for dna fingerprinting.in a search for new fingerprinting probes for use with sheep, repeat sequences derived from five poxviruses, an iridovirus and a baculovirus were screened against dna from sheep pedigrees. probes constructed from portions of the parapox viruses, orf virus and papular stomatitis virus and the baculovirus from the alfalfa looper, autographa californica, nuclear polyhedrosis virus all gave fingerprint patterns. probes from three other poxviruses and an iridovirus did not give useful banding pattern ...19911892249
identification of bluetongue virus vp6 protein as a nucleic acid-binding protein and the localization of vp6 in virus-infected vertebrate cells.recently the insect baculovirus autographa californica nuclear polyhedrosis virus (acnpv) has been effectively adapted as a highly efficient vector in insect cells for the expression of various genes. a cdna sequence of rna segment 9 of bluetongue virus serotype 10 (btv-10, an orbivirus member of the reoviridae family) encoding a minor core protein (vp6) has been inserted into the bamhi site of the pacym1 transfer vector derived from acnpv. spodoptera frugiperda cells were cotransfected with the ...19902152806
genetically engineered multi-component virus-like particles as veterinary vaccines.multiprotein structures can be constructed to mimic virus particles. these engineered particles lack genetic material and are not infectious but they can elicit protective immune responses in animals against challenges with infectious viruses. as a prototype, insect cells were co-infected with two recombinant baculoviruses. one recombinant baculovirus contained an insert of l2 and m5 genes, which encode for outer capsid proteins vp2 and vp5, respectively, from bluetongue virus (btv) downstream o ...19938270267
production of recombinant ovine interferon tau using a bombyx mori nuclear polyhedrosis baculovirus expression system.ovine interferon tau (oifn-tau) is an embryonic protein of critical importance in the establishment of pregnancy in the sheep. we have produced recombinant (r) oifn-tau using a baculovirus expression system and demonstrated the biological activity of the protein produced. bombyx mori larvae were infected with b. mori nuclear polyhedrosis virus (bmnpv), modified by inserting a cdna coding for oifn-tau downstream of the strong polyhedron promoter. following infection, antiviral activity of the hae ...200212212938
baculovirus treatment fully protects mice against a lethal challenge of fmdv.foot-and-mouth disease virus (fmdv) causes a highly contagious and economically devastating disease that affects cattle, swine, goat and sheep among others. fmdv is able to overcome the initial host innate immune response by inhibiting the induction of antiviral molecules at both the transcriptional and the translational levels. it has been demonstrated that fmdv a/arg/2001 causes the death of adult c57bl/6 mice within 72h. we evaluated the capacity of autographa californica nuclear polyhedrosis ...201020580746
the expression and biologic effects of ovine interleukin-4 on t and b cell proliferation.using the reverse-transcriptase polymerase chain reaction (rt-pcr), cdna encoding ovine (ov) interleukin-4 (ovil-4) was generated from mitogen-stimulated peripheral blood mononuclear cells (pbmc). two identical clones generated from separate rt-pcr reactions differed from a published ovil-4 sequence, although they had a high degree of identity with the bovine and human homologs. we show by sequence analysis that the ovil-4 cdna retained the four alpha-helix structure and disulfide bonds identifi ...200010805377
bluetongue virus tubules made in insect cells by recombinant baculoviruses: expression of the ns1 gene of bluetongue virus serotype 10.bluetongue virus (btv) forms tubules in mammalian cells. these tubules appear to be composed of only one type of protein, ns1, a major nonstructural protein of the virus. to obtain direct evidence for the origin of the tubules, the complete m6 gene of btv serotype 10 was inserted into the baculovirus transfer vector pacym1, so that it was under the control of the polyhedrin promoter of autographa californica nuclear polyhedrosis virus. after cotransfection of spodoptera frugiperda cells with wil ...19882845115
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