a 54 kda cysteine protease purified from the crude extract of neodiplostomum seoulense adult a preliminary study for the explanation of pathobiology of neodiplostomum seoulense infection, a 54 kda protease was purified from the crude extract of adult worms by sequential chromatographic methods. the crude extract was subjected to deae-sepharose fast flow column, and protein was eluted using 25 mm tris-hcl (ph 7.4) containing 0.05, 0.1, 0.2 and 0.4 m nacl in stepwise elution. the 0.2 m nacl fraction was further purified by q-sepharose chromatography and protein was eluted using 20 mm s ...199910188382
complement-mediated tail degradation of neodiplostomum seoulense cercariae.the furcocercus cercariae of neodiplostomum seoulense (digenea: neodiplostomidae) penetrate the skins of tadpoles and shed their tails. the speculated mechanism of this tail loss was physical efforts required to produce a vigorous zigzag motion during skin penetration; no other mechanism has been proposed. we examined the relationship between the host serum and cercarial tail loss. cercariae of n. seoulense were collected from experimentally infected segmentina hemisphaerula, and lots of 300 cer ...200616809960
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