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experimental mastitogenicity of mycoplasma capricolum subsp. capripneumoniae for rabbit mammary glands.mycoplasma capricolum subsp. capripneumoniae of cow-udder origin was tested in rabbit mammary-glands for its mastitogenic capability. establishment of mycoplasma organisms and presence of histopathological lesions in mammary glands were the parameters for describing mastitogenic potential. the reisolation of injected mycoplasma capricolum subsp. capripneumoniae organisms in the pure form from the infected glands along with the occurrence of histopathological changes were suggestive of mastitis d ...19989717452
characterization of strains of mycoplasma mycoides subsp. mycoides small colony type isolated from recent outbreaks of contagious bovine pleuropneumonia in botswana and tanzania: evidence for a new biotype.four strains of mycoplasma mycoides subsp. mycoides small colony type (mmmsc) isolated from recent outbreaks of contagious bovine pleuropneumonia (cbpp) in africa have been investigated. one botswanan strain, m375, displayed numerous and significant phenotypic differences from both contemporary field isolates and older field and vaccine strains (african, australian, and european strains dating back to 1936). differences include altered morphology, reduced capsular polysaccharide production, high ...200010747118
specific real-time pcr assays for the detection and quantification of mycoplasma mycoides subsp. mycoides sc and mycoplasma capricolum subsp. capripneumoniae.contagious bovine pleuropneumonia and contagious caprine pleuropneumonia are two severe respiratory infections of ruminants due to infection by mycoplasma mycoides subsp. mycoides sc (mmmsc) and mycoplasma capricolum subsp. capripneumoniae (mccp), respectively. they are included in the oie list of notifiable diseases. here we describe the development of rapid, sensitive, and specific real-time pcr assays for the detection and quantification of mmmsc and mccp dna. mmmsc pcr primers were designed ...200818678244
molecular analysis of field strains of mycoplasma capricolum subspecies capripneumoniae and mycoplasma mycoides subspecies mycoides, small colony type isolated from goats in tanzania.a molecular analysis of strains of mycoplasma capricolum subsp. capripneumoniae (m. capripneumoniae) and mycoplasma mycoides subsp. mycoides, small colony type (m. mycoides sc) isolated from goats was performed using the amplified fragment length polymorphism (aflp) and pulsed-field gel electrophoresis (pfge) fingerprinting techniques. among the 11 field strains of m. capripneumoniae from tanzanian goats, two aflp patterns were demonstrated, with 10 of the strains showing indistinguishable patte ...200111423192
mycoplasmas isolated from the respiratory tract of cattle and goats in tanzania.a microbiological study of the mycoplasma flora in the respiratory tracts of cattle and goats in selected regions of tanzania is described. in the examination of cattle, mycoplasmas were isolated from 60 (17.8%) of the 338 examined lung samples, 8 (47.1%) of the 17 lymph nodes, 4 (13.3%) of the 30 pleural fluid samples and 4 (3.9%) of the 103 nasal swabs examined. all the isolates were identified as mycoplasma mycoides subsp. mycoides, small colony type except for one isolate from pleural fluid ...200011126579
rapid detection of contagious caprine pleuropneumonia using a mycoplasma capricolum subsp. capripneumoniae capsular polysaccharide-specific antigen detection latex agglutination test.latex microspheres (diameter, 8 microm) were coated with anti-mycoplasma capricolum subsp. capripneumoniae polyclonal immunoglobulin g (igg) antiserum (anti-f38 biotype). the coated microspheres, when used in a latex agglutination test (lat), detected m. capricolum subsp. capripneumoniae antigen in the serum of goats with contagious caprine pleuropneumoniae (ccpp). beads also agglutinated strongly in the presence of purified m. capricolum subsp. capripneumoniae capsular polysaccharide (cps). pre ...200011060083
the pathogenicity and pathogenesis of mycoplasma capricolum subsp. capripneumoniae (f38) in the caprine mammary gland.the right mammary gland of 12 lactating goats was inoculated intracisternally with 1 ml of mycoplasma capricolum subsp. capripneumoniae (mcc) containing 10(6) colony-forming units (cfu), while their left mammary halves received 1 ml of sterile pplo broth only. two goats served as uninfected controls. the clinical mastitis that developed in the infected mammary halves within 24 h was initially acute but became increasingly chronic by the end of the experiment at 24 days post inoculation (dpi). th ...19989618887
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