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isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses.seven rotavirus strains were isolated in cell cultures from the intestinal contents of piglets with diarrhea. ma104 cells with pancreatin in the cell culture medium was the host system of choice for virus isolation and replication. a cell culture immunofluorescence test in which ma104 cells were used in microtiter plates was very effective for detecting and assaying rotaviruses. a plaque reduction neutralization test, cross-protection studies in gnotobiotic pigs, and electrophoresis of rotaviral ...19846321541
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