Publications

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serological investigations on the presence of antibodies to tick-borne encephalitis virus in domestic animals and birds and in humans.the presence of hemagglutination-inhibiting antibodies to tick-borne encephalitis virus was investigated in serum samples from domestic animals (217 from swine, 214 from cattle, 179 from goats and 161 from sheep) and birds (214 from geese and 171 from ducks), as well as from 511 apparently healthy subjects of a biotope with particular ornithological and entomological characteristics. the proportion of positive reactions was very low in cattle (1.4%) and goats (1.1%) and more elevated in humans ( ...19854071975
[experimental study of the interrelations of the tick-borne encephalitis virus and vertebrates. 1. large and medium-sized mammals (a review of the literature)]. 19836350835
prevalence of arbovirus antibodies in sera of animals in sri lanka.the sera of cattle, goats, dogs and crows from the colombo area were tested for antibodies against seven arboviruses of the families togaviridae and bunyaviridae by a plaque-reduction neutralization microtest, using vero cells and a stable line of pig kidney (ps) cells. the overall percentages of positive sera among the mammals were: bhanja, 92.5%; calovo (batai), 30.6%; sindbis, 13.8%; langat, 4.8%; tahyna, 3.9%; west nile, 1.6%. among the birds, 23.8% had antibodies to bhanja virus and 9.5% to ...19836609014
consistent patterns in the contact of domestic animals with tick-borne encephalitis virus in the eastern part of the russian plain.hemagglutination inhibition test (hit), neutralization test (nt) and complement fixation test (cft) were used to serologically examine goats and cows in the southern area of the kirov region and in the udmurt assr. a total of 1641 sera of goats and 1414 sera of cows were examined for the presence on antibodies against tick-borne encephalitis virus. the tests showed a relatively intensive contact of goats and cows with tbe virus in the forests of the eastern part of the russian plain: hit was pos ...19846715878
the virus causing encephalomyelitis in sheep in spain: a new member of the tick-borne encephalitis group.the nucleotide and deduced primary amino acid sequence of the envelope gene of two virus isolates from the brains of spanish sheep with encephalomyelitis, were determined and compared with those of other flaviviruses. the amino acid alignments showed that the spanish viruses shared 95 to 96 per cent homology with the envelope protein of louping ill virus and western european tick-borne encephalitis virus. in comparison, the maximum variation in amino acid identities among strains of louping ill ...19957709053
family outbreak of alimentary tick-borne encephalitis in slovakia associated with a natural focus of infection.a family outbreak of tick-borne encephalitis involving 7 people, all of them hospitalized, was observed in the district of povazská bystrica (central slovakia). the disease was associated with the drinking of unboiled goat milk and tick-borne encephalitis virus was recovered from ixodes ricinus ticks collected from places where goats were grazing.19968891541
tick-borne encephalitis virus within and outside japan: a cause for concern? 200212082310
prevalence of tick-borne-encephalitis virus antibodies in lithuania.clinical infections caused by tick-borne encephalitis virus (tbev) are quite common in lithuania and cause significant disease burden not only as acute cases but as chronic sequealeae as well. in order to evaluate the spread of the disease and risk factors, a population based seroprevalence study was done.200212126718
tick-borne encephalitis virus foci in slovakia.tick-borne encephalitis (tbe) virus as a typical arbovirus relies on two types of hosts for its survival: ticks act both as virus vectors and reservoir hosts, and vertebrates amplify the virus infection by acting as a source of infection for feeding ticks. longitudinal monitoring of tbe virus in ticks and vertebrate hosts including humans over a period of 40 years resulted in the identification of the areas of slovakia where tbe virus is endemic. these are concentrated to the western, southern, ...200212141756
[tick borne zoonosis: selected clinical and diagnostic aspects].tick-borne zoonotic infections are among the most diffuse vector borne diseases: these large group of infections is caused by different microorganisms: babesia spp., borrelia spp., rickettsia spp., ehrlichia spp., francisella tularensis, coxiella burnetii) and tick-borne encephalitis virus. babesiosis is caused by the protozoa (sporozoa) babesia microti and it is quite rare in humans in europe. the ixodids ticks are the competent vectors. a few symptomatic cases have been reported, mainly in spl ...200415305697
high seroprevalence of granulocytic ehrlichiosis distinguishes sheep that were the source of an alimentary epidemic of tick-borne encephalitis.a sheep herd from which contaminated cheese was produced, causing 21 cases of alimentary tick-borne encephalitis (tbe) in human beings, was tested serologically for the presence of specific antibodies against both the tbe virus (tbev) and anaplasma phagocytophilum, the cause of tick-borne fever (tbf) in ruminants, and compared with three other herds variously exposed to tick bites but without any tbe history. virus-neutralisation (vn) with the tbev strain hypr and cv-1 cells was used in tbe test ...200415515879
tick-borne encephalitis virus infections in lithuanian domestic animals and ticks.in order to obtain information about the distribution of tick-borne encephalitis virus (tbev) in lithuania, sera of domestic animals were screened for tbev antibodies by haemagglutination inhibition test. samples were collected in 2001 from 423 cows, 561 goats and 118 sheep during a prophylactic examination or vaccination by a local veterinary specialist. in addition, a total of 3234 ixodes ricinus ticks in 436 pools were collected and tested by rt-pcr for the presence of tbev rna (detailed anal ...200516191893
genetic study of the distribution of greek goat encephalitis virus in greece.tick-borne encephalitis virus (tbev) and louping ill virus (liv) are viruses in the mammalian tick-borne virus group/genus flavivirus, causing central nervous system disease. greek goat encephalitis virus (ggev), which was isolated from the brain of a newborn goat with neurological symptoms, is currently classified in the tbev group. the vector of ggev has not yet been specifically identified but is considered likely to be ixodes ricinus. a total of 3,144 ixodidae ticks collected during 2003-200 ...200818471057
early detection of tick-borne encephalitis virus spatial distribution and activity in the province of trento, northern italy.new human cases of tick-borne encephalitis (tbe) have recently been recorded outside the recognised foci of this disease, i.e. in the province of trento in northern italy. in order to predict the highest risk areas for increased tbe virus activity, we have combined cross-sectional serological data, obtained from 459 domestic goats, with analysis of the autumnal cooling rate based on moderate resolution imaging spectroradiometer (modis) land surface temperature (lst) data. a significant relations ...200718686242
tick-borne encephalitis from eating goat cheese in a mountain region of austria.we report transmission of tick-borne encephalitis virus (tbev) in july 2008 through nonpasteurized goat milk to 6 humans and 4 domestic pigs in an alpine pasture 1,500 m above sea level. this outbreak indicates the emergence of ticks and tbev at increasing altitudes in central europe and the efficiency of oral transmission of tbev.200919861072
can goats be used as sentinels for tick-borne encephalitis (tbe) in nonendemic areas? experimental studies and epizootiological observations.a goat flock grazing year-round on a meadow in a "tbe non-risk area" in thuringia, germany, with a history of only isolated human tbe cases was examined repeatedly for tbe virus-(tbev)-specific antibodies and tbev rna between october 2008 and december 2009. surprisingly, tbev specific antibodies were detected in one goat, which had never left this area. to compare the results of a natural contact to tbev with a defined contact to tbev, two goats were immunized experimentally. both animals develo ...201021141272
prevalence of tick-borne encephalitis virus (tbev) in samples of raw milk taken randomly from cows, goats and sheep in eastern poland.a total of 119 unpasteurized milk samples taken from 63 cows, 29 goats and 27 sheep bred on 8 farms situated on the territory of the lublin province (eastern poland), an area of risk of tick-borne encephalitis (tbe), were examined for the presence of rna of tick-borne encephalitis virus (tbev) by the nested rt-pcr method. milk samples were also tested for the presence of anti-tbev antibodies by elisa test. by rt-pcr, the greatest prevalence of tbe virus was found in the milk of sheep (22.2%), fo ...201021186771
experimental infection of goats with tick-borne encephalitis virus and the possibilities to prevent virus transmission by raw goat milk.objectives: the aim of this work was to study the tick-borne encephalitis virus (tbev) infection of goats and the possibilities to prevent human milk-borne infections either by immunizing animals or the heat treatment of milk. methods: an experiment was conducted with 20 milking goats. ten goats (half of them immunized) were challenged with live tbev and 10 were left uninfected. clinical signs and body temperatures of the animals were recorded and milk samples were collected daily. the presence ...201121325791
evaluation of serological tests for detecting tick-borne encephalitis virus (tbev) antibodies in animals.tick-borne encephalitis (tbe) in animals is not well understood yet. tbe virus (tbev) serology in several host species could be valuable for epidemiological analyses in the field as well as for the detection of clinical cases. however, performance and suitability of the available test systems are not well assessed. therefore, we evaluated two commercial tbev-elisa kits in a pilot study and compared them for their suitability in veterinary applications. for this purpose, we tested 163 field colle ...201122191165
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