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isolation of ovine rotavirus in cell cultures. brief report.three cytopathic rotavirus strains were isolated in ma 104 cells from intestinal contents of lambs with diarrhea. pretreatment of virus with pancreatin and incorporation of a small amount of pancreatin in maintenance medium were important for establishment of the strains in these cells. the isolates showed marked cross reactions with bovine rotavirus in immunofluorescence, but no cross reaction with bovine, human, simian, equine, porcine and lapine rotaviruses in neutralization.19852982347
detection and quantitation of rotavirus using monoclonal antibody coupled red blood cells: comparison with elisa.a total of 125 faecal extracts from infants were tested by reverse passive haemagglutination (rph) using red cells coated with a monoclonal antibody against the major group-specific rotavirus antigen (vp 6). results were compared with those obtained using a rabbit anti-rotavirus capture, guinea pig anti-rotavirus detector-based elisa. the specificity of the assay was confirmed by use of 'normal' immunoglobulin coupled red cells and by inhibition with rabbit antiserum. the antibody-coated red cel ...19852997254
enzootic bovine rotavirus is not a source of infection in panamanian cattle ranchers and their families.vaccination of humans against rotavirus (rv) diarrhea may be accomplished by oral immunization with attenuated animal strains known to be antigenically very similar to human strains. to define better the degree of infectivity in nature of these animal strains for humans, we conducted surveillance for rv infection/diarrhea in 180 farm workers, their 161 family contacts, and the 566 animals (512 cattle, 35 pigs, and 19 sheep) on 14 farms in rural panama. no correlation between the high infection r ...19863009644
bovine rotavirus serotypes and their significance for immunization.neutralization assays on calf fecal rotavirus with antisera to two previously described bovine rotavirus serotypes allowed the isolation of four rotaviruses belonging to a distinct third serotype. in a survey of 85 calf isolates, 80 rotaviruses belonged to serotype 1 (91%), 1 belonged to serotype 2 (1%), and 4 belonged to serotype 3 (5%). serotypes 1 and 2 were shown to not cross-protect in a passive immunization experiment in gnotobiotic lambs. ingestion of specific antiserum protected against ...19846092421
isolation and characterization of an equine rotavirus.a rotavirus, designated as the h-1 strain, was isolated from a diarrheic foal in primary african green monkey kidney cells and ma104 cells. this cell culture-adapted strain hemagglutinated erythrocytes of human group o, rhesus monkeys, guinea pigs, and sheep. it was found to be similar, if not identical, to porcine rotaviruses (strains osu, ee, and a-580) by plaque reduction neutralization and hemagglutination inhibition tests, and, in addition, it was found to belong to subgroup 1. this equine ...19836313746
detection of bovine coronavirus in feces by reversed passive hemagglutination.a reversed passive hemagglutination (rpha) method was developed for the detection of bovine coronavirus in fecal specimens. sheep erythrocytes fixed with glutaraldehyde, and then treated with tannic acid were coated with anti-bovine coronavirus rabbit antibodies purified by affinity chromatography using bovine coronavirus linked to sepharose 4b. the rpha test was carried out by a microtiter method. erythrocytes coated with purified specific antibodies were agglutinated by bovine coronavirus, but ...19846367710
prevalence of neutralizing antibodies to 9 rotavirus strains representing 7 g-serotypes in sheep sera.neutralizing antibodies to 9 rotavirus strains representing serotypes g1, g3, g5, g6, g8, g9, and g10 were investigated in 212 ovine serum samples from 3 age groups, 1-week-old lambs, 2- to 3-months-old lambs and adult sheep. all sera from 1-week-old lambs had neutralizing antibodies to all 9 rotavirus strains. both neutralizing antibody titers and prevalences to all 9 strains markedly decreased in the 2- to 3-months-old lamb group and increased again in the adult sheep group. also, adult sheep ...19957483248
genomic characterization of a novel group a lamb rotavirus isolated in zaragoza, spain.an ovine rotavirus (ovr) strain, 762, was isolated from a 30-day-old lamb affected with severe gastroenteritis, in zaragoza, spain, and the vp4, vp7, vp6, nsp4, and nsp5/nsp6 genes were subsequently characterized molecularly. strain ovr762 was classified as a p[14] rotavirus, as the vp4 and vp8* trypsin-cleavage product of the vp4 protein revealed the highest amino acid (aa) identity (94% and 97%, respectively) with that of the p11[14] human rotavirus (hrv) strain pa169, isolated in italy. analy ...200818661221
are human p[14] rotavirus strains the result of interspecies transmissions from sheep or other ungulates that belong to the mammalian order artiodactyla?a limited number of human g6p[14] rotavirus strains that cause gastroenteritis in humans have been isolated in europe and australia. the complete genome sequences were determined for five of these human strains--b10925-97 (isolated in belgium in 1997), 111/05-27 (italy, 2005), pa169 (italy, 1987), mg6 (australia, 1993), and hun5 (hungary, 1997)--and their genetic relatedness to animal rotavirus strains was evaluated by sequencing the complete genome of the sheep rotavirus ovr762 (g8p[14]; spain, ...200919153225
[sequence and evolutionary analysis of vp1 gene of ovine rotavirus nt].the ovine rotavirus strain nt isolated from diarrhea lamb in china was considered as a promising vaccine strain. based on the vp1 gene was one of the important structural proteins of rotavirus, we studied on the evolutional characteristics of vp1.200919835167
ovine rotavirus strain llr-85-based bovine rotavirus candidate vaccines: construction, characterization and immunogenicity evaluation.group a bovine rotaviruses (brvs) are the most important cause of diarrheal diseases in neonatal calves and cause significant morbidity and mortality in the young animals, and epidemiologic surveillance of bovine rotavirus g genotypes conducted in various cattle populations throughout the world has shown that approximately 90% of the bovine rotavirus isolates belong to g6 and g10. based on the modified jennerian approach to immunization, we constructed and characterized a reassortant rotavirus s ...201020488633
differential display analysis of gene expression during the induction of mucosal immunity.one approach to understanding the physiologically relevant events during the induction of an immune response is to identify genes that are expressed when the immune system first encounters antigen. such an investigation requires a naive but fully functional immune system, and the fetal lamb provides these conditions during the last trimester of gestation. 'intestinal segments,' containing a jejunal peyer's patch, were surgically prepared in fetal lambs (>120 days gestation) and individual 'intes ...200011132159
[seroprevalence of viral infections in llamas (lama glama) in the republic of argentina].this study reports the seroprevalence of bovine viral infections in llamas (lama glama) in argentina. this is the first study made in the country including 390 llamas and testing antibodies against eight viruses. samples were collected from nine farms distributed in three different provinces: buenos aires, córdoba and jujuy. the samples were tested for antibodies against eight viruses known to infect cattle: bovine herpesvirus type 1 (bhv-1), bovine viral diarrhea virus (bvdv), bovine adenovirus ...19979229724
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