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virus leakage through natural membrane condoms.the authors determined virus leakage from condoms made from processed sheep caecum using two viral probes simultaneously. they poured a mixture of two viruses, the bacteriophage, phi x174 (4 x 10(7) pfu/ml), and the human pathogen, herpes simplex virus (about 1 x 10(6) pfu/ml), in a buffered solution into condoms, which were suspended into beakers also containing buffered solution. the authors then assayed aliquots from the beakers to measure the extent of virus leakage from the condoms. with on ...19902163114
inability to transmit scrapie by transfection of mouse embryo cells in vitro.infectivity of nucleic acid from highly infectious mouse scrapie brains was studied by transfecting the nucleic acid in vitro prior to inoculation into animals. foetal mouse brain and whole mouse embryo cultures were chosen as the cells used for the transfection. as an internal control, infectious nucleic acid was recovered from bacteriophage phi x174 and whole virus particles were obtained when cultures were transfected with herpes simplex virus dna. in contrast, none of the animals inoculated ...19826278059
mechanism of immune dysfunction associated with minor antigen graft-vs-host disease in mice.a well-characterized murine model of graft-vs-host disease (gvhd) that develops in response to minor histocompatibility antigens was used to study the mechanism of an immunodeficiency syndrome that is associated with gvhd. lethally irradiated mice were transplanted with a combination of bone marrow and spleen cells from h-2 compatible donors that differed at multiple minor histocompatibility antigens, or from syngeneic donors. four to 12 weeks later, the humoral responses of transplanted and con ...19882972364
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