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gen-probe rapid diagnostic system for the mycobacterium avium complex does not distinguish between mycobacterium avium and mycobacterium paratuberculosis.three reference and 16 field strains of mycobacterium paratuberculosis were tested with the gen-probe mycobacterium avium complex dna probe (gen-probe inc., san diego, calif.). all reference strains and 12 of 16 field strains gave positive hybridization results with the probe. this study shows that the m. avium complex probe does not distinguish between m. avium and m. paratuberculosis and indicates heterogeneity in the 16s rrna gene of m. paratuberculosis.19912037681
characterization of a specific mycobacterium paratuberculosis recombinant clone expressing 35,000-molecular-weight antigen and reactivity with sera from animals with clinical and subclinical johne's disease.johne's disease is a chronic enteritis of ruminants associated with enormous worldwide economic losses for the dairy cow- and goat-rearing industries. management limitations and eradication programs for this disease have been hampered by the lack of a simple and specific diagnostic test for the detection of subclinical cases. we used a recombinant clone expressing a 35,000-molecular-weight mycobacterium paratuberculosis antigen (p35 antigen) from a previously constructed expression library of m. ...19979196196
bovine tuberculosis at the wildlife-livestock-human interface in hamer woreda, south omo, southern ethiopia.bovine tuberculosis (btb) is endemic in cattle in the ethiopian highlands but no studies have been done so far in pastoralists in south omo. this study assessed the prevalence of bovine tuberculosis (btb) at an intensive interface of livestock, wildlife and pastoralists in hamer woreda (south omo), ethiopia. a cross-sectional survey including a comparative intradermal skin testing (cidt) was conducted in 499 zebu cattle and 186 goats in 12 settlements. sputum samples from 26 symptomatic livestoc ...201020808913
rapid mycobacterial liquid culture-screening method for mycobacterium avium complex based on secreted antigen-capture enzyme-linked immunosorbent assay.sensors in automated liquid culture systems for mycobacteria, such as mgit, bact/alert 3d, and trek esp ii, flag growth of any type of bacteria; a positive signal does not mean that the target mycobacteria are present. all signal-positive cultures thus require additional and often laborious testing. an immunoassay was developed to screen liquid mycobacterial cultures for evidence of mycobacterium avium complex (mac). the method, called the mac-enzyme-linked immunosorbent assay (elisa), relies on ...200919261776
relationship between is901 in the mycobacterium avium complex strains isolated from birds, animals, humans, and the environment and virulence for poultry.a total of 738 strains of mycobacterium avium complex (mac) were examined in biological experiments on poultry by use of pcr methods with primers for detection of the insertion sequence is901. serotype strains of mac from all known 28 serotypes were examined. further strains were isolated from human immunodeficiency virus (hiv)-negative and hiv-positive patients, 6 animal species, 17 bird species, and the environment. of 165 strains virulent for poultry, characterized by generalized tuberculosis ...200010702495
distribution of serotypes, is901 and a 40 kda protein in mycobacterium avium complex strains isolated from man and animals in denmark.the aim of the present study was to characterize all strains of the mycobacterium avium complex isolated in denmark in 1993. a total of 141 m. avium complex strains (86 from man, 38 from animals, and 17 from peat) were analysed by serotyping, elisa specific for a 40 kda protein, and is901-specific pcr. serotype analysis showed that the most frequent serotypes among human strains were serotype 4 (27%) and serotype 6 (19%), which differs from an earlier survey where serotype 1 was most prevalent. ...19979164469
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