Publications

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a cloned dna probe for cowdria ruminantium hybridizes with eight heartwater strains and detects infected sheep.the dna probe pcs20, which was cloned from the dna of the crystal springs heartwater strain from zimbabwe, cross-reacted with dnas of heartwater strains from all endemic areas, including four heartwater strains from zimbabwe, two strains from south africa, one strain from nigeria, and the gardel strain from the caribbean island of guadeloupe. by nucleic acid hybridization, the pcs20 dna probe detected cowdria ruminantium dna in all dna preparations made from plasma samples from infected sheep be ...19921572987
[ticks and hemoparasitic diseases in cattle in senegal. iv. the southern sudan area].the authors report on the results of an investigation on ticks and hemoparasitoses of cattle, sheep and goats in the south sudanian area of senegal. systematic routine dipping against ticks of cattle, 40 sheep and 40 goats was set during 15 months, with a view to determine the population dynamics together with an acurate localization of the different species concerned. the following parasites were collected from these ruminants: amblyomma variegatum, boophilus geigyi, hyalomma truncatum, h. m. r ...19902218038
[ticks and hemoparasitoses of livestock in senegal. iii. the northern sudan area].the authors describe the results of a study on ticks and hemoparasitoses of cattle and small ruminants in the senegalese north-sudanian area. for 15 months, 40 bovine, 40 sheep and 40 goats received a routine dipping treatment, aimed at the determination of the tick population dynamics together with an accurate localization of the preferential sites for the different species. the following parasites were collected from the animals: hyalomma marginatum rufipes, h. truncatum, rhipicephalus lunulat ...19892485548
babesia, theileria and anaplasma spp. infecting sable antelope, hippotragus niger (harris, 1838), in southern africa.some observations are recorded on blood parasites of sable antelopes. blood smears of 124 of these antelopes from south africa and zimbabwe were examined and 7 were found to be positive for a babesia sp., identified as babesia irvinesmithi martinaglia 1936. a total of 70 of the smears were positive for theilerial piroplasms, while 1 smear had macroschizonts (with cytomeres) and microschizonts of a theileria (= cytauxzoon) sp. one blood smear was positive for an anaplasma sp. attempts to isolate ...19826817250
experimental transmission of babesia bigemina in sheep using infective larval tick of boophilus decoloratus. 19817342213
piroplasms of domestic animals in the macedonia region of greece. 1. serological cross-reactions.during a serological survey on haemoparasites in macedonia, serum samples were collected from cattle, sheep and goats. all sera were tested by the indirect immunofluorescence test (ifat); the cattle sera against theileria orientalis, t. annulata, babesia bigemina, b. bovis, b. divergens and b. major antigens; the sheep and goat sera against t. ovis, b. ovis, b. motasi and b. crassa antigens. parallel tests of negative and positive control sera against all the antigens showed the existence of cro ...19968792579
general review of tick species present in portugal.at present, 24 species are known to occur in portugal: argas vespertilionis, ornithodoros maritimus and ornithodoros erraticus in argasidae; ixodes acuminatus, ixodes bivari, ixodes canisuga, ixodes frontalis, ixodes hexagonus, ixodes ricinus, ixodes simplex simplex, ixodes ventalloi, ixodes vespertilionis, dermacentor marginatus, dermacentor pictus', haemaphysalis hispanica, haemaphysalis inermis, haemaphysalis punctata, rhipicephalus bursa, rhipicephalus pusillus, rhipicephalus sanguineus, rhi ...199911071535
tick-borne diseases in ruminants of central and southern italy: epidemiology and case reports.sera and blood from cattle and sheep were examined for the presence of babesia and theileria spp by microscopy and serology at the parasitology department of the istituto zooprofilattico sperimentale of abruzzo and molise (izsam). of the 47 bovine herds (323 animals) tested, 15 were found positive for babesia bigemina and 1 for babesia bovis. two outbreaks occurred, one caused by b. bigemina and one by b. bovis. the b. bigemina outbreak occurred in abruzzo and has been followed for two years. th ...199911071553
simultaneous detection of anaplasma and ehrlichia species in ruminants and detection of ehrlichia ruminantium in amblyomma variegatum ticks by reverse line blot hybridization.the detection of anaplasma and ehrlichia species is usually based on species-specific pcr assays, since no assay is yet available which can detect and identify these species simultaneously. to this end, we developed a reverse line blot (rlb) assay for simultaneous detection and identification of anaplasma and ehrlichia species in domestic ruminants and ticks. in a pcr the hypervariable v1 region of the 16s ribosomal rna (rrna) gene was amplified with a set of primers unique for members of the ge ...200212243899
incidence and prevalence of tick-borne haemoparasites in domestic ruminants in ghana.giemsa-stained thin blood smears prepared monthly from cattle, sheep and goats in the greater accra region of ghana between may 1994 and december 1996 were examined for presence of tick-borne haemoparasites. the majority of animals were less than 2 months old at the start of the survey. monthly and cumulative incidences are presented of anaplasma sp., babesia bigemina, borrelia sp., eperythrozoon sp., theileria mutans and theileria velifera in cattle, anaplasma sp., borrelia sp., and theileria s ...200415350659
babesia spp. identified by pcr in ticks collected from domestic and wild ruminants in southern switzerland.concurrent infections with vector-borne pathogens affected a cattle herd in switzerland, and one of the pathogens was identified as babesia bigemina, which had never been observed in this country before. therefore, a survey of the occurrence of ruminant babesia spp. and their tick vectors in switzerland was conducted. a total of 2,017 ticks were collected from sheep, goats, cattle, and wild ruminants (deer, roe deer, and chamois) in southern parts of switzerland and identified morphologically. t ...200617021198
detection and quantification of anaplasma marginale dna in blood samples of cattle by real-time pcr.a taqman-based real-time pcr assay was developed for the diagnosis of anaplasma marginale infection of cattle. the established assay was proven to be highly specific, since no cross-reactions were observed with other anaplasma species of ruminants, including the closely related anaplasma centrale, or other haemoparasites of ruminants (anaplasma bovis, anaplasma ovis, anaplasma phagocytophilum, babesia bovis, babesia bigemina, theileria annulata and theileria buffeli). the detection limit was equ ...200717466470
an epidemiological survey on bovine and ovine babesiosis in kurdistan province, western iran.the aim of this study was to determine the prevalence of the babesia infection in domestic animals in kurdistan province of iran for the first time. in this survey, 9,111 domestic livestock, including cattle and sheep, were randomly sampled and examined from 500 flocks in kurdistan province from july 2007 to september 2009. thin peripheral blood smears were taken and then stained by giemsa staining method. from a total of 9,111 collected samples, 2,642 were sheep and 6,469 were cattle. babesia s ...201222109588
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