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plasmid profile analysis, phage typing, and antibiotic sensitivity of salmonella dublin from clinical isolates in the united states.one hundred clinical isolates of salmonella choleraesuis subsp. choleraesuis serovar dublin (salmonella dublin) were examined for phage sensitivity, antibiotic resistance patterns, and plasmid content. computer analysis of the lysis patterns observed by using 27 typing phages divided the s. dublin isolates into 26 groups. one lytic pattern (designated pattern 16) contained 52% of the isolates examined whereas 16 isolates had unique patterns, and nine patterns had fewer than ten members. although ...19921514237
antimicrobial resistance in salmonella enterica subsp. enterica serovar typhimurium from humans and production animals.we have studied the frequency of antimicrobial resistance and epidemiological relatedness among 473 isolates of salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium) from human and veterinary sources. the human strains were clinical isolates from patients with diarrhoea sent to the state serum institute during august 1993 (228 isolates). the animal strains were isolated from clinical or subclinical infections in cattle (48 isolates), pigs (99 isolates) or poultry (98 isolates) ...19979249206
risk factors for clinical salmonella enterica subsp. enterica serovar typhimurium infection on dutch dairy farms.risk factors for outbreaks in 1999 of clinical salmonella enterica subsp. enterica serovar typhimurium infection on dairy farms were studied in a matched case-control study with 47 case farms and 47 control farms. all 47 case farms experienced a clinical outbreak of salmonellosis which was confirmed with a positive bacteriologic culture for serovar typhimurium in one or more samples. serovar typhimurium phage type 401 and 506 (definitive type 104, dt104) were the most frequently isolated phage t ...200212069778
inhibition of foodborne bacteria by the lactoperoxidase system in a beef cube system.biopreservatives are being developed to inhibit the growth of foodborne pathogens and thus improve food safety. the lactoperoxidase system (lps) is a naturally occurring system that has potential for use as an antimicrobial agent in foods. growth of single strains of the pathogens staphylococcus aureus, listeria monocytogenes, escherichia coli o157:h7, salmonella enterica subsp. enterica serovar typhimurium, yersinia enterocolitica, pseudomonas aeruginosa and beef microflora were assessed on lps ...200414967562
dissemination of salmonella enterica subsp. enterica serovar typhimurium var. copenhagen clonal types through a contract heifer-raising operation.salmonella enterica subsp. enterica serovar typhimurium var. copenhagen isolates from a heifer-raising operation and from 11 dairy herds that had their calves contracted to the heifer-raising operation were examined for their phenotypic and genotypic characteristics. results of the study showed that the heifer-raising operation could serve as a clearinghouse for salmonella serovar typhimurium var. copenhagen and perhaps other salmonella serotypes.200516081980
use of antibiotic susceptibility patterns and pulsed-field gel electrophoresis to compare historic and contemporary isolates of multi-drug-resistant salmonella enterica subsp. enterica serovar newport.recently, multi-drug-resistant (mdr) salmonella enterica subspecies enterica serovar newport reemerged as a public and animal health problem. the antibiotic resistance of 198 isolates and the pulsed-field gel electrophoresis patterns (pfge) of 139 isolates were determined. serovar newport isolates collected between 1988 and 2001 were included in the study. one hundred seventy-eight isolates were collected from the san joaquin valley in california and came from dairy cattle clinical samples, huma ...200414711658
antimicrobial susceptibilities of salmonella strains isolated from humans, cattle, pigs, and chickens in the netherlands from 1984 to 2001.we monitored antimicrobial susceptibility data for salmonella strains isolated from humans, cattle, pigs, and chickens in the netherlands from 1984 to 2001 in order to provide insight into the dynamics of resistance over time. the strains were tested for their susceptibilities to seven antimicrobial agents by the agar diffusion method. resistance was most common in salmonella enterica subsp. enterica serovar typhimurium. among the strains from humans, pigs, and chickens, it was found that the le ...200312904357
occurrence of salmonella enterica serovar dublin in austria.in austria, salmonella enterica subsp. enterica serovar dublin, a bovine-adapted serovar, rarely causes infections in humans. in 2000, austria was within the european mean with an incidence of 0.1 per million inhabitants. our data show that the vast majority of all serovar dublin infections (human and non-human) can be traced epidemiologically to two districts in the tyrol. this concentration of cases can be explained by a particularly traditional aspect of cattle farming in this area, the alpin ...200212631940
effect of previous antimicrobial treatment on fecal shedding of salmonella enterica subsp. enterica serogroup b in new york dairy herds with recent clinical salmonellosis.the association of herd- and sample-level factors with the isolation of salmonella group b from cattle fecal samples was analyzed. study farms were 65 dairy herds with a recent history of laboratory-confirmed clinical salmonella infections. herds were visited once per month for three months to collect data and samples for bacteriological culture. herd size varied widely from 34 to 3700 total cattle on the farm (median=370). salmonella serogroup b was isolated from 270 of 2726 samples tested. the ...200312507855
a multiplex real-time pcr assay targeting virulence and resistance genes in salmonella enterica serotype typhimurium.abstract:201121707966
subtyping of salmonella typhimurium by pulsed-field gel electrophoresis and comparisons with phage types and resistance types.one-hundred and sixty-eight salmonella enterica subsp. enterica serotype typhimurium isolates have been analysed by phage typing, by pulsed-field gel electrophoresis and for their antimicrobial susceptibility. those independent strains, isolated from food animal production including cattle, poultry and pig sectors have been collected by the french non human salmonella network, during the first semester in 1999. isolates encompassed 14 phage types. the majority of s. typhimurium isolates was foun ...200212171058
probability of and risk factors for introduction of infectious diseases into dutch spf dairy farms: a cohort study.a 2-year cohort study was conducted to investigate the probability of disease introduction into dutch dairy farms. the farms were tested regularly for diseases and were visited biannually to collect management data. ninety-five specific pathogen-free (spf) dairy farms were selected from a database of bovine herpesvirus type 1 (bhv1)-free farms to study the probability of, and risk factors for, introduction of bhv1, bovine viral diarrhoea virus (bvdv), salmonella enterica subsp. enterica serotype ...200212114014
salmonella serotypes isolated from nonhuman sources in são paulo, brazil, from 1996 through 2000.a total of 4,581 salmonella strains isolated from nonhuman sources, including foodstuffs associated with foodborne salmonella outbreaks, from january 1996 through december 2000 were serotyped at the enteropathogens laboratory, instituto adolfo lutz, são paulo, brazil. among the 123 different serotypes identified, salmonella enterica subsp. enterica serotype enteritidis (salmonella enteritidis) was the most prevalent (32.7%), ranking first for almost every kind of source. the next most common ser ...200212092719
herd-level diagnosis for salmonella enterica subsp. enterica serovar dublin infection in bovine dairy herds.herd-level sensitivities of bacteriological and serological methods were compared in 79 bovine dairy herds, recently infected with salmonella enterica subsp. enterica serovar dublin. all farms experienced clinical signs of salmonellosis for the first time and had no history of vaccination against salmonellosis. at the start of the study, infection with serovar dublin was confirmed with at least one positive bacteriologic culture for serovar dublin from a clinical case (gold standard for herd inf ...200211821135
evaluation of two enzyme-linked immunosorbent assays for detecting salmonella enterica subsp. enterica serovar dublin antibodies in bulk milk.two enzyme-linked immunosorbent assays (elisas) for the detecting salmonella enterica subsp. enterica serovar dublin antibodies in bulk milk were developed and evaluated for potential use in control programs. the elisas were based on either lipopolysacharide (lps elisa) or flagellar antigen (gp elisa). sensitivity was determined with 79 case herds with a wide range of clinical signs. specificity was determined with 125 dutch and 200 swedish control herds. the relation between antibodies in bulk ...200111687438
evaluation of three newly developed enzyme-linked immunosorbent assays and two agglutination tests for detecting salmonella enterica subsp. enterica serovar dublin infections in dairy cattle.in this study test characteristics of three newly developed enzyme-linked immunosorbent assays (elisas) for salmonella enterica subsp. enterica serovar dublin were evaluated and compared with two agglutination tests. the elisas involved were an indirect elisa with serovar dublin lipopolysaccharide (lps elisa), an indirect elisa with serovar dublin flagellar antigen (gp elisa), and a double-antibody sandwich blocking elisa that uses monoclonal antibodies against s. enterica subsp. enterica serova ...200011101571
evaluation of is200-pcr and comparison with other molecular markers to trace salmonella enterica subsp. enterica serotype typhimurium bovine isolates from farm to meat.a procedure that uses an original molecular marker (is200-pcr) and that is based on the amplification of dna with outward-facing primers complementary to each end of is200 has been evaluated with a collection of 85 salmonella enterica subsp. enterica serotype typhimurium isolates. these strains were isolated from a group of 10 cows at different stages: during transportation between the farm and the slaughterhouse, on the slaughter line, from the environment, and from the final product (ground be ...200010834977
attachment of salmonella choleraesuis choleraesuis to beef muscle and adipose tissues.the attachment of salmonella choleraesuis subsp. choleraesuis atcc 15790 to beef muscle and adipose tissues was investigated. s. choleraesuis was found to adhere in higher numbers to muscle than to fat. the charge and the hydrophobicity of the surface of s. choleraesuis were evaluated by measurement of electrophoretic mobility, the contact angle with water, adhesion to hexadecane, and hydrophobic interaction chromatography. the overall negative charge of s. choleraesuis was masked by the high el ...199710465034
increasing incidence and comparison of nalidixic acid-resistant salmonella enterica subsp. enterica serotype typhimurium isolates from humans and animals.we determined the resistance to quinolone of 309 salmonella enterica subsp. enterica serotype typhimurium strains isolated from humans and animals (cattle, pigs, or poultry) in 1995 or 1996. nalidixic acid resistance increased from 8.5% in 1995 to 18.6% in 1996. the highest resistance levels correlated with a mutation at ser-83 (or asp-82). all strains remained ciprofloxacin susceptible. human and animal isolates were compared by pulsed-field gel electrophoresis, and the banding patterns of the ...19999854111
evaluation and comparison of molecular techniques for epidemiological typing of salmonella enterica subsp. enterica serovar dublin.a total of 28 unrelated isolates of the salmonella enterica subsp. enterica serovar dublin (s. dublin) collected during a 6-year period, as well as four samples of the s. dublin live vaccine strain bovisaloral and its prototype strain s. dublin 442/039, were investigated by different molecular typing methods for the following reasons: (i) to find the most discriminatory method for the epidemiological typing of isolates belonging to this salmonella serovar and (ii) to evaluate these methods for t ...19968904430
geographical and temporal dissemination of salmonellae isolated from domestic animal hosts in the culiacan valley, mexico.the prevalence and diversity of salmonellae from domestic animal hosts were investigated in the culiacan valley, mexico. a total of 240 farm animal feces (cows, chicken, and sheep) were evaluated for salmonella spp. presence from july 2008 to june 2009. salmonella enterica subsp. enterica strains were isolated from 76 samples (31.7%), and 20 serotypes were identified being salmonella oranienburg (25%), salmonella give (14%), salmonella saintpaul (12%), and salmonella minnesota (11%) the most fre ...201121274525
salmonella enterica subsp. enterica in cattle egret (bubulcus ibis) chicks from central texas: prevalence, serotypes, pathogenicity, and epizootic potential.cattle egrets have a worldwide distribution, feed in proximity to cattle and other domestic animals, and often nest in large colonies in urban woodlots. over a 3-yr period, nestlings from five cattle egret colonies from central texas, usa, were surveyed for salmonellosis. prevalence of infection ranged from 29% to 95%. seventeen salmonella enterica subsp. enterica serotypes were isolated, of which the 4,5,12:i-monophasic serotype predominated in cultures of both the digestive tract and pooled sp ...201020688631
the prevalence of multidrug resistance is higher among bovine than human salmonella enterica serotype newport, typhimurium, and 4,5,12:i:- isolates in the united states but differs by serotype and geographic region.salmonella represents an important zoonotic pathogen worldwide, but the transmission dynamics between humans and animals as well as within animal populations are incompletely understood. we characterized salmonella isolates from cattle and humans in two geographic regions of the united states, the pacific northwest and the northeast, using three common subtyping methods (pulsed-field gel electrophoresis [pfge], multilocus variable number of tandem repeat analysis [mlva], and multilocus sequence ...201020639364
effect of bovine lactoferrin in salmonella ser. typhimurium infection in mice.lactoferrin (lf) has in vitro antimicrobial activity against gram-negative bacteria. salmonella enterica subsp. enterica serovar typhimurium causes systemic infection and acute diarrhea in humans, mainly in children younger than 2 years of age. the aim of the study was to determine the in vivo effect of bovine lf in salmonella ser. typhimurium infection in mice. 58 balb/c mice were employed. two hours before the infection with 300 microl of 10(7) cfu of salmonella ser. typhimurium, 29 mice recei ...201020306285
[study on the epidemiological characteristics and molecular typing of salmonella enterica subsp. enterica serovar senftenberg in shanghai].to study the molecular epidemiological characteristics of salmonella enterica subsp. enterica serovar senftenberg (salmonella senftenberg) in shanghai, from 2006 to 2007.200920193231
pulsed-field gel electrophoresis diversity of human and bovine clinical salmonella isolates.pulsed-field gel electrophoresis (pfge) characterization of 335 temporally and spatially matched clinical, bovine, and human salmonella enterica subsp. enterica isolates revealed 167 xbai pfge patterns. these isolates were previously classified into 51 serotypes and 73 sequence types, as determined by multilocus sequence typing. discriminatory power of pfge (simpson's index, d = 0.991) was considerably higher than that of multilocus sequence typing (d = 0.920) or serotyping (d = 0.913). although ...201020180633
environmental sampling to predict fecal prevalence of salmonella in an intensively monitored dairy herd.although dairy cattle are known reservoirs for salmonellae, cattle that are shedding this organism are often asymptomatic and difficult to identify. a dairy herd that was experiencing a sustained, subclinical outbreak of salmonella enterica subsp. enterica cerro was monitored for 2 years. fecal samples from the lactating cows were collected every 6 to 8 weeks and tested for the presence of salmonella. fecal prevalence of salmonella fluctuated throughout the observation period and ranged from 8 t ...200818939739
longitudinal study of a clonal, subclinical outbreak of salmonella enterica subsp. enterica serovar cerro in a u.s. dairy herd.salmonellae are a major group of foodborne pathogens known to affect both humans and animals. dairy cattle are a known reservoir of these bacteria and human salmonella infections have been associated with the consumption of improperly processed or contaminated dairy products. many of the over 2500 known serotypes of salmonella are known to infect cattle, resulting in asymptomatic to fatal salmonellosis. this study describes the course of a salmonella outbreak and subsequent endemic infection on ...200718041954
isolation of salmonella enterica serotype newport from a partly ruptured splenic abscess in a traveler returning from zanzibar.salmonella enterica subsp. enterica serotype newport is a pathogen of growing importance because of its epidemic spread in dairy cattle and increasing rate of antimicrobial resistance. human infections, however, are rare. we report a case of a splenic abscess in a young traveler returning from east africa.200717634302
simulation model estimates of test accuracy and predictive values for the danish salmonella surveillance program in dairy herds.the danish government and cattle industry instituted a salmonella surveillance program in october 2002 to help reduce salmonella enterica subsp. enterica serotype dublin (s. dublin) infections. all dairy herds are tested by measuring antibodies in bulk tank milk at 3-month intervals. the program is based on a well-established elisa, but the overall test program accuracy and misclassification was not previously investigated. we developed a model to simulate repeated bulk tank milk antibody measur ...200616979767
culling decisions of dairy farmers during a 3-year salmonella control study.salmonella enterica subsp. enterica-serotypes lead to periodically increased morbidity and mortality in cattle herds. the bacteria can also lead to serious infections in humans. consequently, denmark has started a surveillance and control programme in 2002. the programme focuses on salmonella dublin which is the most prevalent and most persistent serotype in the danish cattle population. a field study in 10 dairy herds with persistent salmonella infections was carried out over three years to gai ...201121481960
onset of diarrhea and pyrexia and time to detection of salmonella enterica subsp enterica in feces in experimental studies of cattle, horses, goats, and sheep after infection per os.to determine time to first detection of salmonella organisms in feces of animals after experimental infection po and times to onset of diarrhea and pyrexia to evaluate a common method for identifying nosocomial infections on the basis of time of admission and onset of clinical signs (ie, the 3-day criterion).201121568780
multi-locus sequence typing of salmonella enterica subsp. enterica serovar enteritidis strains in japan between 1973 and 2004.salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis) was responsible for a worldwide pandemic during the 1980s and 1990s; however, changes in the dominant lineage before and after this event remain unknown. this study determined s. enteritidis lineages before and after this pandemic event in japan using multilocus sequence typing (mlst). thirty s. enteritidis strains were collected in japan between 1973 and 2004, consisting of 27 human strains from individual episodes, a bovi ...201121672260
specific detection of campylobacter jejuni using the bacteriophage nctc 12673 receptor binding protein as a probe.campylobacter jejuni is found in the intestines of poultry, cattle, swine, wild birds and pet animals and is the major cause of foodborne gastroenteritis in developed countries. we report the use of the receptor binding protein (rbp) of campylobacter bacteriophage nctc 12673 for the specific capture of campylobacter jejuni bacteria using rbp-derivatized capturing surfaces. the gp48 rbp was expressed as a glutathione s-transferase-gp48 (gst-gp48) fusion protein and immobilized onto surface plasmo ...201121955997
molecular follow-up of salmonella enterica subsp. enterica serovar agona infection in cattle and humans.salmonella enterica subsp. enterica serovar agona was not frequently encountered in finland until an increase in rates of isolation among animal and feed was seen in 1994. a small outbreak among cattle farms in the regions of oulu and vaasa in northwestern finland in 1994-1995 included eight farms. after the outbreak, an increase in the number of serovar agona infections in humans was seen in 1999: the number of annual microbiologically confirmed cases in humans increased from about 10 from 1990 ...200212354860
Prevalence of Salmonella enterica and the hygienic indicator Escherichia coli in raw meat at markets in Ouagadougou, Burkina Faso.This study investigated the hygienic status and prevalence of Salmonella and Escherichia coli in retail meat sold at open markets in Ouagadougou, Burkina Faso. A total of 150 samples of beef meat (n = 45), beef intestine (n = 45), mutton (n = 30), and chicken (n = 30) were collected from four local markets for investigation. The prevalence of Salmonella enterica subsp. enterica was 9.3%, and six serotypes, all previously unreported in Burkina Faso, were identified: Derby, Tilene, Hato, Bredeney, ...201121902926
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