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chemical and biological properties of endotoxin from leptospira interrogans serovars canicola and icterohaemorrhagiae.1. endotoxin-like activity was extracted with phenol-chloroform-petroleum either (pcp) from leptospira interrogans serovars icterohaemorrhagiae and canicola. chemical analysis of leptospiral cells obtained from the pcp extract indicated the following distribution of lipopolysaccharide (lps), protein and polysaccharide in mg/ml: 3.0, 4.5 and 1.0 for icterohaemorrhagiae and 3.3, 5.6 and 1.5 for canicola. 2. the preparations presented several biological activities: positive limulus test (1.0 pg/ml) ...19921342222
[actual problems of leptospirosis in animals in poland]. 19883247482
hemolytic activity of leptospira interrogans serovar canicola cultured in protein-free medium.hemolytic activity of the culture supernatant of leptospira interrogans serovar canicola strain moulton grown in protein-free medium was demonstrated. the activity began to appear in the late logarithmic phase of growth of the organism and reached a plateau after 2 weeks of cultivation. it was inactivated by the addition of dipotassium ethylenediaminetetraacetate, but was effectively restored by mg2+. hemolysis by the culture supernatant was stimulated by "hot-cold" incubation. sleep erythrocyte ...19826813653
biological activities of lipopolysaccharide-like substance (lls) extracted from leptospira interrogans serovar canicola strain moulton.the biological activities of lipopolysaccharide-like substance (lls) extracted from leptospira interrogans serovar canicola strain moulton by the hot phenol-water method were studied in mice. the addition of 12.5 micrograms/ml or more of lls fraction increased the incorporation of [3h]thymidine into in vitro cultured spleen cells of c57bl/6 mice, while the activity of the lls fraction was about 20 times weaker than that of salmonella typhimurium lipopolysaccharide (lps). pretreatment of murine s ...19873500390
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