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prevention of human rotavirus-induced diarrhea in gnotobiotic piglets using bovine antibody.the efficacy of passively administered bovine antibody for preventing human rotavirus (hrv)-induced diarrhea was investigated using a gnotobiotic pig model. cows were immunized with inactivated hrv serotypes 1 (wa) and 2 (s2) and simian rotavirus serotype 3 (sa11), and immune colostrum and milk were collected. antibody concentrates derived from these materials were fed to germ-free piglets that were subsequently inoculated with hrv wa. both viral shedding and diarrhea were effectively reduced or ...19921313067
expression of the gene coding for the major outer capsid protein of sa-11 rotavirus in a baculovirus system.the gene coding for the major outer capsid protein (vp7) of simian rotavirus sa-11 has been expressed in a baculovirus-insect cell system. the resulting protein is 35 kda and is primarily associated with the endoplasmic reticulum. neutralizing sa-11 polyclonal antiserum and vp7 monospecific antiserum reacted specifically with the expressed gene product. antiserum derived against the recombinant vp7 protein neutralized sa-11 rotavirus infectivity in a fluorescent focus assay.19921318623
protection of agammaglobulinemic piglets from porcine rotavirus infection by antibody against simian rotavirus sa-11.rotavirus, a double-stranded rna virus, has been implicated as a diarrhea-provoking agent in a variety of animal species. several previous reports have shown that immunization with a single serotype may result in increased in vitro neutralization titers against serotypes not represented in the immunogen. this study was undertaken to determine whether antibody from cows immunized against simian rotavirus strain sa-11 (which is alien to pigs) could protect neonatal piglets from infection with a no ...19911653265
vp4 monotype specificities among porcine rotavirus strains of the same vp4 serotype.the porcine rotavirus osu strain was used to produce monoclonal antibodies (mabs) directed against the outer capsid protein vp4. from two separate fusions, eight mabs that inhibited hemagglutination activity of the osu strain were selected. all mabs immunoprecipitated both the osu vp4 protein derived from a lysate of infected ma104 cells and the osu vp4 protein expressed in sf9 cells by a recombinant baculovirus. by immunoprecipitation of in vitro-translated osu gene 4 transcripts of different l ...19911847483
genetic relatedness among animal rotaviruses.the genomic relatedness among representative rotavirus strains was examined by employing cross-hybridization techniques. single stranded (ss) rna prepared by in vitro transcription of purified rotavirus particles and labeled with either 32p or 125i was hybridized to denatured genomic, double stranded (ds) rnas. the hybrids formed were analyzed by polyacrylamide gel electrophoresis (page) or by testing their sensitivity to digestion with single strand specific nuclease (s-1 nuclease). a relativel ...19863004389
a comparison of rotavirus strains of bovine, simian and porcine origin.three rotavirus strains of bovine, simian and porcine origin, respectively, were compared. the 3 viruses induced a classic rotaviral infection in newborn, conventionally reared calves. the cross neutralization tests revealed an antigenic identity of simian and porcine rotaviruses and a slight serologic correlation of these two viruses with the bovine rotavirus strain. however, in reciprocal cross protection tests carried out in calves, the simian rotavirus antiserum afforded weak protection to c ...19853021518
localization of rotavirus antigens in infected cells by ultrastructural immunocytochemistry.virus structural antigens were localized within a line of monkey kidney (ma104) cells infected with the simian rotavirus sa11 using electron microscopic immunoperoxidase techniques. when hyperimmune guinea-pig anti-sa11 serum was used, virus particles, membranes of virus-associated endoplasmic reticulum, and viroplasmic inclusions were most heavily labelled. a general cytoplasmic reaction (ribosomes, intracytoplasmic membranes, etc.) with anti-sa11 serum was also observed, but nuclei were unstai ...19826296288
characterization of temperature-sensitive mutants of simian rotavirus sa11: protein synthesis and morphogenesis.the synthesis of viral polypeptides, distribution of viral antigens, and morphogenesis of viral structures have been examined in cells infected with temperature-sensitive (ts) mutants of sa11 representing 10 recombination groups. at the permissive temperature (31 degrees c) the synthesis of viral polypeptides and the distribution of viral antigens did not differ significantly from those of the wild type. at the nonpermissive temperature (39 degrees c) some mutants (tsb, -c, -e, -f, and -g) synth ...19846321755
genetic stability of a porcine rotavirus rna segment during repeated plaque isolation.the porcine group a rotavirus cc86 was characterized to explore its utility as a tool for mutation analysis. it has a semiduplication of the gene 11 rna segment. nucleotide sequence determination of cdna confirmed that the nsp5 coding sequence and the conserved nontranslated termini of the rna segment were retained. a comparison of the nsp5 genes of cc86 and cn86 that were isolated from the same fecal specimen showed eight base pair changes, suggesting that cn86 was not the immediate progenitor ...19968918545
[vp4 protein of simian rotavirus strain sa11 expressed by a baculovirus recombinant].the complementary dna copies of the gene 4 were prepared by reverse transcription and polymerase chain reaction (rt-pcr) from genomic rna of simian rotavirus strain sa11. a complete vp4 gene was been inserted into a baculovirus intermediate vector pvl-1393 which was under control of the polyhedrin promoter. the outer capsid protein vp4 which was a major neutralization antigen of rotavirus, was expressed in high yield in spodoptera frugiperda cell line. the vp4 protein expressed was recognized wi ...199710453552
the stability of porcine rotavirus in feces.rotaviruses are known as major causal agents of diarrhea in humans and animals. they affect young animals in intensive rearing and cause great economic losses. this study evaluated the infectivity of porcine rotavirus maintained for 32 months at approximately 10 degrees c in the original stool specimens. thirty stool specimens of 1-4-week-old piglets from breeding farms located in the southwest of the state of parana were selected for this study. they were randomly chosen from stool samples posi ...200010665529
comparative sequence analysis identified mutations outside the nsp4 cytotoxic domain of tissue culture-adapted atcc-wa strain of human rotavirus and a novel inter-species variable domain in its c-terminus.complete nucleotide sequence of the tissue culture-adapted atcc-wa strain of human rotavirus nsp4 was determined. sequence analysis detected two alternate forms of the gene with a nucleotide difference at position 331 (a or g) in the coding sequence (nsp4-a or nsp4-g) leading to a change from neutral glutamine97 in nsp4-a to a positively charged arginine97 in nsp4-g originating from the same atcc-wa preparation. in addition to this, both forms of atcc-wa nsp4 revealed three mutations at nucleoti ...200011043941
a rotavirus strain isolated from pig-tailed macaque (macaca nemestrina) with diarrhea bears a p6[1]:g8 specificity.a distinct rotavirus strain (ptrv) was isolated in cell cultures from a stool sample obtained from a diarrheic 3-year-old female pig-tailed macaque (macaca nemestrina) that was born at the breeding colony of the university of washington in seattle. unlike other known simian rotavirus strains including vervet monkey rotavirus sa11 which bears p5b[2]:g3 or p6[1]:g3 specificity, rhesus monkey rotavirus mmu18006 with p5b[3]:g3 specificity, pig-tailed macaque rotavirus yk-1 with p[3]:g3 specificity a ...200616242747
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