infection of bovine fetuses at 120 days' gestation with virulent and avirulent strains of bluetongue virus serotype 11.bluetongue virus infection in sheep and cattle during fetal development causes neuropathology. two strains of bluetongue virus serotype 11 designated as uc-2 and uc-8 have different virulence patterns in newborn mice. these viruses have distinctly different electropherotype patterns on polyacrylamide gel electrophoresis indicating a genetic difference in these two viruses of the same serotype. four bovine fetuses each were inoculated intramuscularly with either uc-2 or uc-8, and one fetus was in ...19921312421
isolation and identification of a variant of bluetongue virus serotype 11 from a ram in a bluetongue outbreak in western texas.a field strain of bluetongue virus was isolated from a blood sample of a ram during an outbreak of bluetongue in november 1985 in western texas. in this bluetongue outbreak at least 25 of the 2,000 sheep were infected. isolation was made by intravenous inoculation of 11-day-old embryonated chicken eggs. the serotype was identified as serotype 11 by serum neutralization tests. the genomic pattern on sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis (page) of the new isolate is simil ...19911653269
possible introduction of epizootic hemorrhagic disease of deer virus (serotype 2) and bluetongue virus (serotype 11) into british columbia in 1987 and 1988 by infected culicoides carried on the wind.outbreaks of epizootic hemorrhagic disease of deer and of bluetongue began in british columbia in august and october 1987 respectively and recrudescence of infection by both viruses was detected the following year in august. weather records for up to 18 days before the initial outbreaks of disease, isolation of virus or seroconversion were examined to determine if the viruses could have been introduced by infected culicoides carried on the wind. data on temperature, rainfall, wind speed and dire ...19911665099
genetic variation of bluetongue virus serotype 11 isolated from host (sheep) and vector (culicoides variipennis) at the same site.genetic relatedness of 2 strains of bluetongue virus (btv) serotype 11 that were isolated from the same geographic site--one from host (sheep) and the other from the vector culicoides variipennis during an enzootic of bluetongue at bruneau, idaho, in august 1973--was determined by comparing the oligonucleotide fingerprint analyses of the individual double-stranded rna segments of the genomes. it was observed that the 2 strains of btv-11 exhibit considerable differences in their genotypes, the pe ...19836307090
effects of temperature on virogenesis of bluetongue virus serotype 11 in culicoides variipennis sonorensis.culicoides variipennis sonorensis females were fed bluetongue virus serotype 11 mixed in sheep blood and were held at constant temperatures of 32, 27, 21 and 15 degrees c. virogenesis, as measured by enzyme-linked immunosorbent assay (elisa), proceeded significantly faster at higher temperatures. based on elisa absorbance > or = 0.2, some flies first were categorized as infected after 1 day, 2 days and 4 days at 32, 27 and 21 degrees c, respectively. peak levels of virus antigen were seen after ...19957696691
experimentally induced infection with bluetongue virus serotype 11 in cows.the consequences of inoculation of bluetongue virus (btv) serotype 11 into 16 susceptible cows either at the time of breeding or at specified stages of pregnancy were studied. the cows were free of btv or epizootic hemorrhagic disease virus, and none had antibodies to btv before virus inoculation. a group of 4 cows was mated naturally to a bull reported to shed btv-11 (co75b300 strain) in the semen. the bull was suspected of infecting cows at mating with btv-11, which subsequently transplacental ...19947879975
molecular epidemiology of bluetongue virus in northern colorado.the molecular epidemiology of bluetongue virus serotype 11 (btv11) in an enzootic focus in northern colorado was investigated. viruses isolated up to 12 years apart, from both vertebrate and invertebrate hosts, were compared by phylogenetic analysis of nucleotide sequence data from three genome segments: l2, s7, and s10. for each segment, viruses isolated from ruminants in the 1980s were more similar to one another than to viruses isolated from culicoides spp. insects in the 1990s. nearly identi ...200616337708
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