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genetic and biological characterization of tick-borne encephalitis virus isolated from wild rodents in southern hokkaido, japan in 2008.tick-borne encephalitis virus (tbev) is a zoonotic agent causing severe encephalitis in humans. a recent epizootiological survey indicated that endemic foci of tbev have been maintained in the southern part of hokkaido until recently. in this study, we sought to isolate tbev from wild rodents in the area. one virus, designated oshima 08-as, was isolated from an apodemus speciosus captured in hokuto in 2008. oshima 08-as was classified as the far eastern subtype of tbev and formed a cluster with ...201323590320
isolation of tick-borne encephalitis virus from wild rodents and a seroepizootiologic survey in hokkaido, japan.to determine the vertebrate host of tick-borne encephalitis (tbe) virus in the southern part of hokkaido, japan, virus isolation was performed using spleens from small mammals captured in the area. two virus strains were isolated, one strain from apodemus speciosus and another from clethrionomys rufocanus. virus isolates were inoculated onto baby hamster kidney cell monolayers and antigen slides were prepared for an indirect immunofluorescent antibody assay. two isolates were identified as tbe v ...199910072153
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