Publications

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host-range restriction of chimeric yellow fever-west nile vaccine in fish crows (corvus ossifragus).we evaluated a recombinant virus chimera, chimerivax-wn, in which the west nile virus (wnv) surface protein genes (pre-membrane [prm] and envelope [e]) are substituted into the genome of the 17d vaccine strain yellow fever virus (yf-17d), as a vaccine candidate for protection of birds from wnv disease. using fish crows (corvus ossifragus) as a model, we found that none of eight crows that received two high doses of vaccine (approximately 100,000 plaque-forming units [pfu]) developed viremia and ...200312932102
dna vaccine for west nile virus infection in fish crows (corvus ossifragus).a dna vaccine for west nile virus (wnv) was evaluated to determine whether its use could protect fish crows (corvus ossifragus) from fatal wnv infection. captured adult crows were given 0.5 mg of the dna vaccine either orally or by intramuscular (im) inoculation; control crows were inoculated or orally exposed to a placebo. after 6 weeks, crows were challenged subcutaneously with 105 plaque-forming units of wnv (new york 1999 strain). none of the placebo inoculated-placebo challenged birds died. ...200314519243
vectest as diagnostic and surveillance tool for west nile virus in dead birds.the vectest antigen-capture assay for west nile virus was performed on oral and tissue swabs from dead birds in new york state from april 2003 through july 2004. results were compared with those from real-time reverse transcriptase-polymerase chain reaction of kidney or brain. oral vectest sensitivity is adequate for surveillance in american crows (corvus brachyrhynchos) (87%), blue jays (cyanocitta cristata) (80%), and house sparrows (passer domesticus) (76%). oral vectest performed well for sm ...200415663856
west nile virus quantification in feces of experimentally infected american and fish crows.to better understand the potential environmental health risk presented by west nile virus (wnv)-contaminated feces, we quantified the amount of wnv present in the feces of experimentally infected american crows (corvus brachyrhynchos) and fish crows (corvus ossifragus). peak fecal titers ranged from 10(3.5) to 10(8.8) plaque-forming units (pfu)/g for 10 american crows and from 10(2.3) to 10(6.4) pfu/g for 10 fish crows. the presence of infectious wnv in bird feces indicates a potential for direc ...200617038695
west nile virus antibody prevalence in american crows (corvus brachyrhynchos) and fish crows (corvus ossifragus) in georgia, usa.crows have been the centerpiece of avian west nile virus (wnv) surveillance and research in north america. this work has demonstrated variation in susceptibility to wnv infection between american (cor vus brachyrhynchos) andfish crows (corvus ossifragus). the higher wnv-associated mortality rate in american crows compared with fish crows suggests that wnv antibody prevalence would be greater in the fish crow population. the objectives of this study were to 1) determine whether fish crows had hig ...200717461278
declining mortality in american crow (corvus brachyrhynchos) following natural west nile virus infection.the american crow (corvus brachyrhynchos) is known to suffer 100% mortality from infection with the new york 1999 strain of west nile virus (wnv). following the initial detection of wnv in north america in 1999, we measured prevalence of wnv-reactive antibodies ("seroprevalence") in free-ranging american and fish crows (corvus ossifragus) of central new jersey after each transmission season through 2005. in 2002, seroprevalence in american crow juveniles increased to 14% from the 5% of the previ ...200919848089
multiplexed microsatellite loci in american crow (corvus brachyrhynchos): a severely affected natural host of west nile virus.recent advances in high throughput molecular techniques have allowed the development of cost- and time-effective libraries of molecular markers, such as microsatellites, for population genetic studies in non-model species. the american crow, corvus brachyrhynchos, is recognized to be one of the species that has been most negatively affected by the emergence of west nile virus (wnv) in north america in 1999. genetic monitoring of the process of a declining population after the introduction of an ...201222982159
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