Publications

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vector roles of fennoscandian mosquitoes attracted to mammals, birds and frogs.1. mosquitoes were sampled with five suction traps, from may to october 1983, at a forest site in south-central sweden. 2. twenty-three species of mosquitoes were identified among the total of 3108 females collected: 4% of them in an unbaited trap, 3% in a trap baited with two frogs, 24% with a guinea-pig, 28% with a hen and 40% in a trap baited with a rabbit. 3. the dominant species of culicidae trapped were 39% aedes communis (de geer), 21% ae. cinereus meigen, 14% coquillettidia richiardii (f ...19901983455
overwintering of culex mosquitoes in sweden and their potential as reservoirs of human pathogens.1. culex pipiens l. and cx torrentium martini are suspected enzootic vectors in sweden of sindbis virus causing ockelbo disease in man. to estimate their potential as virus reservoirs over the winter, mosquitoes were collected from august to may from a cellar in a rural area, central sweden. the mosquitoes were dissected and examined for blood content, gonotrophic stage, parity and insemination, and tested for fructose. 2. cx pipiens, cx torrentium and cx territans walker constituted 94% of 887 ...19872856507
seasonal activity of mosquitoes in an ockelbo disease endemic area in central sweden.in the sässman area, province of hälsingland, sweden, mosquitoes were sampled in 1983 from aquatic habitats, overwintering sites, cattle sheds, human bait, with uv-light traps, rabbit-, guinea-pig-, hen-, dove- and unbaited suction traps and similarly baited net traps. anopheles beklemishevi, an. messeae, aedes cantans, ae. cataphylla, ae. cinereus, ae. communis, ae. diantaeus, ae. excrucians, ae. hexodontus, ae. intrudens, ae. leucomelas, ae. pionips, ae. punctor, ae. vexans, culex pipiens, cx. ...19862906960
diel activity patterns of blood-seeking anthropophilic mosquitoes in central sweden.1. blood-seeking diel activity cycles of mosquitoes are described from 24 h landing catches on human bait in a field and a forest habitat during june-august in central sweden. 2. daytime activity in the field was low or absent, whilst in the forest activity was relatively even throughout the diel. 3. in both habitats, activity peaks generally occurred about sunset and sunrise and were more distinct in the field than in the forest. 4. data from this and other studies show that, in general, aedes ...19882908783
an assessment of culex pipiens quinquefasciatus say as a vector of virus diseases in the witwatersrand region of the transvaal. ii. sindbis virus. 19665953933
mosquito sensitivity to a scorpion neurotoxin expressed using an infectious sindbis virus vector.the scorpion, androctonus australis hector, produces an insect-specific toxin (aahit) encoded by the scotox gene. to assess the toxicity of aahit for mosquitoes, we have taken a novel approach to express the scotox gene in vivo. we have engineered a double subgenomic sindbis (dssin) virus that contains the scotox gene in the viral genome and intrathoracically inoculated the virus (te/3'2j/scotox) into mosquitoes (aedes aegypti, ae. triseriatus and culex pipiens), houseflies (musca domestica) and ...19957551198
laboratory transmission of sindbis virus by aedes albopictus, ae. aegypti, and culex pipiens (diptera: culicidae).the susceptibility of aedes albopictus (skuse) for sindbis (sin) virus was examined in the laboratory. ae. albopictus, ae. aegypti (l.), and culex pipiens (l.) became infected with and subsequently transmitted sin virus by bite to chicks after feeding on viremic 1-d-old chicks. after ingesting 10(5.3) plaque-forming units (pfu)/ml, ae. albopictus had the highest transmission rate (30%) of the 3 species. transmission by ae. aegypti was less efficient (7%), whereas none of the cx. pipiens transmit ...19958551504
development of a new sindbis virus transducing system and its characterization in three culicine mosquitoes and two lepidopteran species.alphavirus transducing systems (atss) are alphavirus-based tools for expressing genes in insects. here we describe an ats (5'dsmre16ic) based entirely on sindbis mre16 virus. gfp expression was used to characterize alimentary tract infections and dissemination in three culicine and two lepidopteran species. following per os infection, 5'dsmre16ic-egfp efficiently infected aedes aegypti and culex tritaeniorhynchus, but not culex pipiens pipiens. ae. aegypti clearly showed accumulation of green fl ...200414728670
characterization of an endogenous gene expressed in aedes aegypti using an orally infectious recombinant sindbis virus.sindbis virus expression vectors have been used successfully to express and silence genes of interest in vivo in several mosquito species, including aedes aegypti, ae. albopictus, ae. triseriatus,culex pipiens, armigeres subalbatus and anopheles gambiae. here we describe the expression of an endogenous gene, defensin, in ae. aegypti using the orally infectious sindbis virus, mre/3'2j expression vector. we optimized conditions to infect mosquito larvae per os using c6/36ae. albopictus cells infec ...200115455070
trans-packaged west nile virus-like particles: infectious properties in vitro and in infected mosquito vectors.a trans-packaging system for west nile virus (wnv) subgenomic replicon rnas (reprnas), deleted for the structural coding region, was developed. wnv reprnas were efficiently encapsidated by the wnv c/prm/e structural proteins expressed in trans from replication-competent, noncytopathic sindbis virus-derived rnas. infectious virus-like particles (vlps) were produced in titers of up to 10(9) infectious units/ml. wnv vlps established a single round of infection in a variety of different cell lines w ...200415479801
first report on human-biting culex pipiens in sweden.culex mosquitoes are vectors of several bird-hosted arboviruses that cause outbreaks in europe, such as sindbis virus and west nile virus. recently, the human-biting form of culex pipiens, cx. pipiens biotype molestus, was found causing big nuisance in a housing cooperative in gothenburg in southern sweden, confirmed by molecular identification. this is the first report of human-biting culex in scandinavia, signalling increased risk of arbovirus infection in northern europe.201627927233
mosquitoes (diptera: culicidae) and their relevance as disease vectors in the city of vienna, austria.mosquitoes (diptera: culicidae) are important vectors for a wide range of pathogenic organisms. as large parts of the human population in developed countries live in cities, the occurrence of vector-borne diseases in urban areas is of particular interest for epidemiologists and public health authorities. in this study, we investigated the mosquito occurrence in the city of vienna, austria, in order to estimate the risk of transmission of mosquito-borne diseases. mosquitoes were captured using di ...201425468380
wide recognition of culex pipiens and lack of detection of culex torrentium through biomolecular differentiation of mosquitoes in the emilia-romagna region, northern italy.the culex pipiens complex includes species with reported differences in vector competence for arthropod-borne viruses, many of which are of significant importance to human health such as the west nile virus and the sindbis virus. this group of mosquitoes is difficult to distinguish morphologically; particularly as adult females. in europe, the two species of the complex, culex pipiens linnaeus 1758 and culex torrentium martini 1925, are often found sympatrically. with the aim to characterize the ...201627477051
trapping biases of culex torrentium and culex pipiens revealed by comparison of captures in cdc traps, ovitraps, and gravid traps.we evaluate three trapping methods for their effectiveness at capturing culex pipiens and culex torrentium, both enzootic vectors of bird-associated viruses in europe. the comparisons, performed in two regions in sweden, were among cdc traps baited with carbon dioxide, gravid traps, and ovitraps baited with hay infusion. the proportions of the two culex species in a catch differed between trap types, with cdc traps catching a lower proportion of cx. torrentium than both gravid traps and ovitraps ...201526047196
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