clinical and virological observations on swine experimentally infected with getah virus.the pathogenicity of getah virus for swine was examined. all 8 pigs (4 adults and 4 piglets) inoculated with strains mip-99 and mi-110 developed pyrexia ranging from 39.4 to 40.7 degrees c and anorexia. mild depression and diarrhea were observed in 2 of the 4 piglets. these clinical signs were transient. viremia occurred 1-2 days post-inoculation (p.i.) and the maximum titer was 10(3.0) tcid50 0.1 ml-1. the virus was recovered from a piglet autopsied on day 3 p.i. from spleen and various lymph n ...19882836997
mosquitoes and mosquito-borne arboviruses in the qinghai-tibet plateau--focused on the qinghai area, investigation was conducted to identify the distribution of mosquitoes and mosquito-borne arboviruses in the qinghai-tibet plateau, china from july to august in 2007. a total of 8,147 mosquitoes representing six species from three genera (aedes, culex, and anopheles) were collected in three locations (geermu city, altitude of 2,780 m; xining city, 2,200 m; minhe county, 1,700 m). six virus isolates were obtained including tahyna virus (tahv), liaoning virus, and culex pipiens pallens densovir ...201020348523
multiplex pcr and multiplex rt-pcr for inclusive detection of major swine dna and rna viruses in pigs with multiple infections.multiplex pcr and multiplex rt-pcr were developed to identify nine viruses in pigs with multiple infections. these viruses are: porcine circovirus type 2 (pcv2), suid herpesvirus 1, porcine parvovirus (ppv), porcine reproductive and respiratory syndrome virus (prrsv), japanese encephalitis virus, porcine rotavirus a (porv-a), porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev), and getah virus. these methods were shown to be high specificity and sensitivity. in the ...200919467264
genomic analysis of some japanese isolates of getah virus.using the reverse transcription-polymerase chain reaction (rt-pcr) and direct sequencing, capsid protein and non-structural protein 1 (nsp1) regions of sagiyama virus and eight getah virus strains were analysed. the viruses were isolated from malaysia and various areas of japan over a period of 30 years. based on the available published sequence data, oligonucleotide primers were designed for rt-pcr and the sequences were determined. our findings showed that though there were differences in the ...200111557154
enzyme-linked immunosorbent assay for the serological survey of getah virus in pigs. 19902167998
isolation of getah virus from dead fetuses extracted from a naturally infected sow in japan.three viruses producing a cytopathic effect in cell culture were isolated from dead fetuses extracted from a naturally infected sow, and were found to be serologically identical by neutralization tests. one of the viruses was cloned and named the sakura strain. the sakura strain was identified as getah virus by cross-neutralization tests.19911652864
isolation of getah virus from mosquitos collected on hainan island, china, and results of a isolate of getah virus was obtained from culex mosquitos collected in mao'an village, baoting county, hainan province, china, in 1964. the virus (strain m-1) replicated in laboratory-bred aedes aegypti and cx. fatigans (= quinquefasciatus), and was transmitted by laboratory-bred ae. albopictus to healthy newborn albino mice. skeletal muscles of newborn albino mice experimentally infected with the virus showed degeneration, atrophy, necrosis, and inflammatory changes of muscle fibers. antibody ...19921338481
[isolation and identification of arboviruses from mosquito pools in some regions of liaoning province, china].to isolate and identify arboviruses from mosquito pools in some regions of liaoning province.200919565849
serological survey of arthropod-borne viruses among wild boars in japan.a total of 90 blood samples were collected from wild boars in the kyushu region of japan, and a seroepidemiological survey for 7 arthropod-borne viruses was performed using hemagglutination inhibition tests. the individual seropositive rates for each virus were 52.2% for japanese encephalitis virus (jev), 47.8% for getah virus (getv), 13.3% for akabane virus, 10.0% for aino virus and less than 5% for bluetongue virus, chuzan virus and ibaraki virus. the results indicated that among the 7 viruses ...200919721358
viruses isolated from mosquitoes collected in sri lanka.attempts to isolate viruses from 178,181 unengorged female mosquitoes collected from different ecologic areas of sri lanka yielded 31 isolates: 17 of japanese encephalitis (je) virus, nine of getah virus, three of a batai-related bunyavirus, and two of arkonam virus. culex tritaeniorhynchus and mansonia uniformis mosquitoes were found to carry je virus in a dry zone nonepidemic area, and cx. pseudovishnui was found to carry it in a wet zone nonepidemic area. japanese encephalitis virus was isola ...19947915499
experimental infection of getah virus in swine. 19883210480
ross river virus transmission, infection, and disease: a cross-disciplinary review.ross river virus (rrv) is a fascinating, important arbovirus that is endemic and enzootic in australia and papua new guinea and was epidemic in the south pacific in 1979 and 1980. infection with rrv may cause disease in humans, typically presenting as peripheral polyarthralgia or arthritis, sometimes with fever and rash. rrv disease notifications in australia average 5,000 per year. the first well-described outbreak occurred in 1928. during world war ii there were more outbreaks, and the name ep ...200111585790
serological evidence for the transmission of getah virus in hong kong. 19948085315
a fatal case in newborn piglets with getah virus infection: isolation of the virus. 19872828741
a fatal case in newborn piglets with getah virus infection: pathogenicity of the isolate. 19872828730
evaluation of the human host range of bovine and porcine viruses that may contaminate bovine serum and porcine trypsin used in the manufacture of biological products.current u.s. requirements for testing cell substrates used in production of human biological products for contamination with bovine and porcine viruses are u.s. department of agriculture (usda) 9cfr tests for bovine serum or porcine trypsin. 9cfr requires testing of bovine serum for seven specific viruses in six families (immunofluorescence) and at least 2 additional families non-specifically (cytopathicity and hemadsorption). 9cfr testing of porcine trypsin is for porcine parvovirus. recent con ...201122000165
oligonucleotide fingerprint analysis of strains of getah virus isolated in japan and malaysia.eighteen strains of getah virus isolated from mosquitoes, swine and horses in japan (1956 to 1981), and one strain isolated in malaysia (1955), were analysed by rnase t1-resistant oligonucleotide fingerprinting. all fingerprints showed a poly(a) tract. the fingerprint pattern of the malaysian strain was quite different from those of the japanese strains. although most of the recent japanese isolates shared many large oligonucleotide spots in common, the patterns were not identical even among the ...19846094708
isolation and characterization of a sagiyama virus from domestic 2002, a strain of sagiyama virus (sagv) designated ml/taiwan/02 was isolated from farmed pigs in taiwan. the nsp1 and e1 gene sequences of the ml/taiwan/02 strain shared 98.6 and 96.7% homology, respectively, with corresponding genes of a japanese strain of sagv. nucleotide and amino acid sequence comparison revealed this strain of sagv to be most closely related to getah virus, as opposed to its current classification as a subtype of ross river virus. to investigate the seroprevalence of sag ...200616617695
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