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development of a visual loop-mediated isothermal amplification method for rapid detection of the bacterial pathogen pseudomonas putida of the large yellow croaker (pseudosciaena crocea).in recent years, the large yellow croaker (pseudosciaena crocea), an important marine fish farmed in the coastal areas of zhejiang province, east china, has become severely endangered as a result of the bacterial pathogen pseudomonas putida. this paper reports the development of a visual loop-mediated isothermal amplification (lamp) assay for rapid detection of the pathogen. four primers, f3, b3, fip and bip, were designed on the basis of dna sequence of the rpon gene of p. putida. after optimiz ...201222475582
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