use of pcr to identify leptospira in kidneys of big brown bats (eptesicus fuscus) in kansas and nebraska, usa.bats have been implicated as potential carriers of leptospira as a result of surveys, mostly in australia and south america. we measured the prevalence of pathogenic leptospires in kidneys of bats from kansas and nebraska. from 7 august 2012 to 21 august 2012, we extracted dna from kidneys of 98 big brown bats (eptesicus fuscus) submitted and found negative for rabies. the dna was processed in a two-step, seminested pcr assay with a dual-labeled taqman probe specific for pathogenic leptospires. ...201424807182
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