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isolation and characterization of novel human parechovirus from clinical samples.using vero cells, we isolated a virus (nii561-2000) from a cerebrospinal fluid specimen of a 1-year-old girl with reye syndrome. the determined amino acid sequence of the virus indicated that the isolate was a human parechovirus (hpev), a member of picornaviridae. neutralization test showed that the nii561-2000 virus had distinct antigenicity to hpev-1, hpev-2, and hpev-3, and that the sequence was distinct from these types as well as from hpev-4 and hpev-5. thus, we propose the virus (nii561-20 ...200717553229
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