| multiplex pcr detection of enterotoxin genes in aeromonas spp. from suspect food samples in northern taiwan. | aeromonads possess an array of virulence factors and are causative agents of a number of human infections. among them, genes of one cytotoxic (act) and two cytotonic (alt, ast) enterotoxins are implicated in a human diarrheal disease. a rapid, specific, simultaneous detection of these enterotoxin genes in suspected food poisoning samples is not yet reported. hence, a multiplex pcr assay was designed to amplify the cytotoxic (act), heat-labile cytotonic (alt), and heat-stable cytotonic (ast) ente ... | 2008 | 18939759 |
| molecular diversity of class 1 integrons in human isolates of aeromonas spp. from southern taiwan. | this work studies antimicrobial resistance and class 1 integrons of aeromonas spp. in human isolates from southern taiwan. pcr amplification and dna sequence analyses were performed to characterize the gene cassette regions of the class 1 integron in 204 isolates of aeromonas hydrophila, 36 isolates of a. sobria, 23 isolates of a. veronii, and 4 isolates of a. caviae. by using southern hybridization with an inti1 probe to determine the presence of class 1 integrons in the 9 isolates of aeromonas ... | 2008 | 18806339 |