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molecular and immunological detection of bovine herpesvirus-1 in clinical specimens.a nested polymerase chain reaction (nested-pcr), utilizing glycoprotein b (gb) gene of bovine herpesvirus-1 (bhv-1) as a target, virus isolation (vi), indirect immunofluorescence (ifa) and in situ immuno-peroxidase (ipr) staining in cell culture were conducted for detection of bhv-1 in suspected cattle, buffalo and sheep clinical specimens. the specimens were obtained in the form of nasal swabs, buffy coats and sera from different localities in egypt (ei-sharquia, dumyat and ei-fayoum). a total ...200517977217
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