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[cloning, expression of the cp gene and antiserum preparation of zucchini yellow mosaic virus infecting benincasa hispida cogn. var. chieh-qua how].a guangzhou isolate of zymv infecting benincasa hispida cogn. var. chieh-qua how was identified by indicator tests and partial sequence amplification. the coat protein (cp) gene of this virus was amplified by rt-pcr, and ligated to the expression vector pet-22b(+). the recombinant plasmid pet-zcp was transformed into e. coli bl21 (de3) and then induced to express by iptg. it was shown that the cp gene was highly expressed by sds-page and western blot analysis. the molecular weight of the recombi ...200515847180
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