comparison of is1245 restriction fragment length polymorphism and pulsed-field gel electrophoresis for typing clinical isolates of mycobacterium avium subsp avium.little is still known about the epidemiology and pathogenesis of mycobacterium avium subsp avium (masa) infection.200010815741
[avian mycobacteriosis in humans remains a threat in the czech republic].the members of mycobacterium avium complex belong to the most common agents causing mycobacterioses in humans. they are classified as a potentially patogenic mycobacteria, although mycobacterium avium subsp. avium represent veterinary and economic risks in birds (mainly poultry) as well as mammals (pigs etc.). infected animals and their products (mainly eggs) often come from small household production and pose a risk for human health. the second member of this complex, mycobacterium avium subsp. ...201020401832
distribution of mycobacterium avium subsp. avium and m. a. hominissuis in artificially infected pigs studied by culture and is901 and is1245 quantitative real time pcr.mycobacterium avium subsp. avium (maa) and m. a. hominissuis (mah) belong to the mycobacterium avium complex (mac) and are frequently associated with diseases in animals and humans. the aim of this study was to develop a system for rapid and accurate real time quantitative pcr (qpcr) identification and quantification of maa and mah. this study included 22 per os infected pigs, of which 10 were infected with maa, 10 with mah and 2 were present as a negative control group. from each animal, 21 dif ...201020227841
lipopentapeptide induces a strong host humoral response and distinguishes mycobacterium avium subsp. paratuberculosis from m. avium subsp. avium.many non-tuberculous mycobacteria synthesize abundant glycopeptidolipids (gpls). these surface-located gpls are involved in pathogenicity by interfering with the host immune system. in mycobacterium avium subsp. avium (mav), gpls consist of a lipopeptide core composed of a tetrapeptide o-linked to mono- and oligo-saccharides. the biosynthesis pathway of the simplest gpls is now relatively well understood and involves probably more than fifteen genes. whereas it is very obvious that most, if not ...200818068277
comparative evaluation of positive tests to mycobacterium avium subsp. paratuberculosis in clinically healthy sheep and goats in south-west greece using molecular techniques, serology, and culture.mycobacterium avium subsp. paratuberculosis (map) is the cause of paratuberculosis, which affects mainly ruminants although there is a growing concern about its possible implication in crohn's disease in humans especially in connection with environmental spread and risks to the food chain. retail cheese may represent a significant source of human exposure to map and the aim of this study was to assess map status in clinically healthy sheep and goats in greece, comparing techniques routinely used ...200717084095
comparative genomic hybridizations reveal genetic regions within the mycobacterium avium complex that are divergent from mycobacterium avium subsp. paratuberculosis isolates.mycobacterium avium subsp. paratuberculosis is genetically similar to other members of the mycobacterium avium complex (mac), some of which are nonpathogenic and widespread in the environment. we have utilized an m. avium subsp. paratuberculosis whole-genome microarray representing over 95% of the predicted coding sequences to examine the genetic conservation among 10 m. avium subsp. paratuberculosis isolates, two isolates each of mycobacterium avium subsp. silvaticum and mycobacterium avium sub ...200515774884
putative in vitro expressed gene fragments unique to mycobacterium avium subspecies a suppression subtractive hybridization based method, nine novel mycobacterium avium subsp. paratuberculosis (m. paratuberculosis) fragments of between 318 and 596 bp have been identified and characterized. database search revealed little or no similarity with other mycobacteria. the uniqueness and diagnostic potential of seven of these fragments in relation to m. paratuberculosis closest relative mycobacterium avium subsp. avium (m. avium) was confirmed by species-specific pcr and southern b ...200212351231
histologic and genotypic characterization of a novel mycobacterium species found in three cats.three cases of feline atypical mycobacteriosis from different geographical regions in north america were characterized by large clusters of filamentous bacteria visible on hematoxylin-and-eosin-stained tissue sections. pcr amplification demonstrated the presence of mycobacterium-specific nucleic acid in samples of skin lesions from these cases. pcr-assisted cloning and dna sequence analysis of a 541-bp length of the mycobacterium 16s rrna gene generated dna sequences which were >95% identical, s ...200212089257
alkyl hydroperoxide reductases c and d are major antigens constitutively expressed by mycobacterium avium subsp. paratuberculosis.antigens characteristic for mycobacterium avium subspecies paratuberculosis were identified by crossed immunoelectrophoresis (cie) and by absorbing out cross-reactive antigens by using a polyclonal and polyvalent mycobacterium avium subspecies avium antiserum. two antigens were present in m. avium subsp. paratuberculosis and not detected in mycobacterium avium subsp. avium. they were identified as antigens 17 and 20 in a cie reference system for m. avium subsp. paratuberculosis antigens. purifie ...200010639449
molecular epidemiology of mycobacterium avium subsp. avium and mycobacterium intracellulare in captive birds.mycobacterium avium subsp. avium and mycobacterium intracellulare are primary causes of mycobacteriosis in captive birds throughout the world, but little is known about how they are transmitted. to define the local epidemiology of infection, we strain-typed 70 m. avium subsp. avium and 15 m. intracellulare culture isolates obtained over a 4-year period from captive birds. typing was performed using randomly amplified polymorphic dna (rapd) pcr, amplified fragment length polymorphic (aflp) fragme ...200817689893
comparative macrorestriction and rflp analysis of mycobacterium avium subsp. avium and mycobacterium avium subsp. hominissuis isolates from man, pig, and germany, tuberculous lesions in slaughtered pigs due to infection with members of the mycobacterium avium complex are increasingly reported. contaminated food originating from pig or other livestock is discussed as potential source of human infection. m. avium isolates from man (n=45), pig (n=29), and cattle (n=13) were characterised by restriction fragment length polymorphism (rflp) with respect to insertion sequences is1245 and is901 as well as by xbai-based pulsed-field gel electrophoresis ...200616797885
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