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identification, using isoenzyme electrophoresis and monoclonal antibodies, of leishmania isolated from humans and wild animals of ecuador.six strains of leishmania isolated from wild mammals and humans on the pacific coast of ecuador were identified by isoenzyme electrophoresis and by their reactivity patterns to a cross-panel of specific monoclonal antibodies using a radioimmune binding assay. single isolates from sciurus vulgaris, potos flavus, and tamandua tetradactyla were identified as leishmania amazonensis. three other strains, isolated from cutaneous lesions of humans, were identified as leishmania panamensis.19892919726
genetic analysis of leishmania parasites in ecuador: are leishmania (viannia) panamensis and leishmania (v.) guyanensis distinct taxa?in the course of an epidemiologic survey in ecuador, the following collection of leishmania stocks was isolated: 28 from patients with clinical signs of leishmaniasis, 2 from sloths, 1 from a dog, and 4 from sand flies. for genetic characterization of these stocks, multilocus enzyme electrophoresis (mlee) and random amplified polymorphic dna (rapd) were used. twenty six of the 35 stocks were identified as either leishmania (v.) panamensis or l. (v.) guyanensis, 2 stocks were identified as l. (v. ...199910586922
imported new world cutaneous leishmaniasis in a traveller from slovakia.we present a case of imported leishmaniasis in a 31-year-old woman from slovakia, who visited the countries of south america for three months in 2011. on 29 and 31 august 2011, she was probably infected with leishmania parasites in the jungles of ecuador. approximately one week after returning to slovakia, a small papules appeared on patient's left leg. another wound was found after two weeks. both ulcers were enlarging. we proved amastigote forms of leishmania spp. only in repeated dermal scrap ...201525869571
leishmaniasis recidiva cutis due to leishmania (viannia) panamensis in subtropical ecuador: isoenzymatic characterization.information regarding leishmaniasis recidiva cutis (lrc), a clinical variant of cutaneous leishmaniasis, in the new world is scarce. lrc is characterized by slowly progressing lesion(s) that appear after a variable period of time, from months to years, in or around the scar of an apparently clinically healed sore.200616445499
usefulness of sampling with cotton swab for pcr-diagnosis of cutaneous leishmaniasis in the new world.in this study, we tested the polymerase chain reaction (pcr)-method to diagnose cutaneous leishmaniasis (cl) by taking exudate materials from lesions with cotton swabs, using our previously tested (pcr) panel comprised of leishmania (viannia) panamensis, l. (v.) braziliensis, l. (v.) guyanensis, l. (leishmania) mexicana and l. (l.) amazonensis. the objectives of the present study were to improve the sampling method convenient for the patients and to test the usefulness of samples taken with cott ...200211835896
evidence for hybridization by multilocus enzyme electrophoresis and random amplified polymorphic dna between leishmania braziliensis and leishmania panamensis/guyanensis in ecuador.the taxonomic attribution of four leishmania stocks isolated from humans in ecuador has been explored by both multilocus enzyme electrophoresis and random amplified polymorphic dna. for three loci, mlee results showed patterns suggesting a heterozygous state for a diploid organism, while the corresponding homozygous states are characteristic of the leishmania panamensis/guyanensis complex and leishmania braziliensis, respectively. other enzyme loci showed characters attributable to either the l. ...19979304809
[leishmaniasis in ecuador. 6. epidemiological and entomological note on the focus of leishmaniasis in zumba].the zumba focus of tegumentary leishmaniasis lies in the southwards amazonian region of ecuador. a clinico-epidemiological study has been carried out in the area on 83 patients attending health centers. all the biotopes suitable for sandflies, including dwellings, have been sampled from february to september 1992 by light trap and human bait catches. the number of sandflies caught amounts to 2,547. anthropophilic sandfly fauna is poor and only three species have been recorded. lutzomyia serrana ...19948024349
[leishmaniasis in ecuador. 5. leishmaniasis and anthropization on the pacific coast].we have evaluated the impact of anthropization of the forest on the incidence of leishmaniasis, due to leishmania panamensis, in three coastal study areas, corriente grande (primary forest), paraiso escondido and la tablada (secondary forest). the situation of isolated dwellings, in deforested areas, has also been analysed in the last two stations. in each station, the study of the density of anthropophilic sand flies, specially lutzomyia trapidoi, has been conducted in the domestic environment, ...19948024348
[leishmaniasis in ecuador. 4. natural infestation of the dog by leishmania panamensis].in two endemic leishmaniasis foci of the pacific coast of ecuador 34 dogs suspected of having the disease have been surveyed clinically, serologically and parasitologically; immunofluorescence and electrosyneresis tests, lymph node aspirates, biopsies and smears have been performed. from two dogs with ulcers only one had ulcers on the muzzle and the scrotum infected by leishmania (l. guyanensis complex). the isolated strain was identified as leishmania panamensis. the disease was strictly cutane ...19948024347
[leishmaniasis in ecuador. 3. lutzomyia trapidoi, vector of leishmania panamensis].lutzomyia trapidoi, the more abundant anthropophilic species, is a presumed leishmaniasis vector in the pacific foothills of ecuador. three biotopes have been sampled (dwelling, and nearby coffee crop and primary forest) in the focus of paraiso escondido, by human bait catches, from august 1991 to october 1992. a large number of sandflies, 6,965 specimens, have been dissected to estimate peri and hypopyloric infections. all the peripyloric infections, characterized by isoenzyme electrophoresis, ...19948024346
[leishmaniasis in ecuador. 1. incidence of cutaneous leishmaniasis on the pacific coast].a clinico-epidemiologic survey on cutaneous leishmaniasis, due to leishmania panamensis, was carried out on 961 persons in two study areas of the pacific coast of ecuador, to estimate the prevalence and the incidence of the disease. in the preandean hills, at paraiso escondido, the prevalence of active lesions was 4.8%; in the hills of the coastal cordillera, at la tablada, it was 3.6%. the incidence of new cases in 1991 was high: 147% in the first village, and 106% in the second. these data are ...19948024344
molecular karyotype characterization of leishmania panamensis, leishmania mexicana, and leishmania major-like parasites: agents of cutaneous leishmaniasis in ecuador.molecular karyotypes of leishmania isolates from patients with cutaneous leishmaniasis in ecuador were analyzed by pulsed-field gel electrophoresis (pfge) and southern blot hybridization. the dna karyotypes of l. major-like parasites were similar between two human isolates from a lowland coastal and a highland andean region, but were apparently different from those of eleven world health organization reference strains including l. major. the smallest chromosome of 240 kilobases in l. major-like ...19938517490
atypical clinical variants in new world cutaneous leishmaniasis: disseminated, erysipeloid, and recidiva cutis due to leishmania (v.) panamensis.in recent times, there has been an increase in the number of reports for new and rare variants of cutaneous leishmaniasis (cl). here, we describe three unusual clinical forms of cl identified in ecuadorian children. a total of 131 patients with cl were diagnosed over a 2-year period of active search. in 3 (2.29%), the lesions were very unusual; these included erysipeloid, recidiva cutis (lrc), and disseminated leishmaniasis (dl). the erysipeloid case is characterized by erythematous and indurate ...200516103590
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