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monoclonal antibody-based serological assays and immunocapture-rt-pcr for detecting rice dwarf virus in field rice plants and leafhopper vectors.rice dwarf virus (rdv) causes rice dwarf disease, which leads to considerable losses in rice production in asia. purified rdv virions were used as the immunogen to prepare monoclonal antibodies (mabs). three murine mabs against rdv were prepared. plate-trapped antigen enzyme-linked immunosorbent assay (pta-elisa), dot enzyme-linked immunosorbent assay (dot-elisa) and immunocapture-rt-pcr (ic-rt-pcr) were then developed for sensitive, specific, and rapid detection of rdv in rice and leafhopper sa ...201424121134
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