| aedes aegypti (l.) and aedes albopictus (skuse) in singapore city. 5. observations in relation to dengue haemorrhagic fever. | dengue haemorrhagic fever in singapore was a disease of the urban human population, with concentrations of cases occurring in areas of high population density. mosquito surveys revealed that these areas also had high population densities of ae. aegypti and ae. albopictus.the disease occurred throughout the year but the incidence of cases appeared to follow a seasonal pattern. observations from 1966 to 1968 showed that the number of cases increased in april, reached a peak in november, and, there ... | 1971 | 5316749 |
| monitoring of dengue viruses in field-caught aedes aegypti and aedes albopictus mosquitoes by a type-specific polymerase chain reaction and cycle sequencing. | virologic surveillance for dengue through the detection of the prevalent serotype(s) circulating in the human population during inter- and intra-epidemic periods constitutes a reliable sentinel system for dengue outbreaks. we have applied a rapid and sensitive, semi-nested, reverse transcription-polymerase chain reaction (rt-pcr) assay using nonstructural protein 3 gene primers for the type-specific-detection of dengue viruses in artificially infected and in field-caught adult aedes mosquitoes. ... | 1998 | 9598444 |
| the effect of temperature and humidity on dengue virus propagation in aedes aegypti mosquitos. | the effect of temperature and relative humidity on dengue virus propagation in the mosquito as one of the possible contributing factors to dengue hemorrhagic fever (dhf) outbreaks was studied. ae. aegypti mosquitos were reared under standard conditions and inoculated intrathoracically with dengue virus. virus propagation in the mosquitos was determined at the temperature and relative humidity of all 3 seasons of yangon and for the simulated temperature and relative humidity of singapore. the vir ... | 1998 | 9886113 |
| detection of dengue viruses in field caught male aedes aegypti and aedes albopictus (diptera: culicidae) in singapore by type-specific pcr. | field male aedes aegypti (l.) and aedes albopictus (skuse) adults caught from fixed monitoring stations weekly for 1 yr were screened for dengue viruses (den-1, den-2, den-3, and den-4). the assay was carried out using a single-step reverse transcription (or transcriptase)-polymerase chain reaction (pcr) (rt-pcr) followed by a semi-nested pcr using an upstream consensus primer and four type-specific primers within the nonstructural protein three gene (ns3) of dengue viruses. the diagnostic fragm ... | 2001 | 11476326 |
| dengue virus infection rate in field populations of female aedes aegypti and aedes albopictus in singapore. | we developed a single-step reverse transcription-polymerase chain reaction (rt-pcr) followed by a semi-nested pcr using an upstream consensus primer and four type-specific primers within the non-structural protein gene (ns3) of dengue viruses to type dengue viruses in field populations of female aedes mosquitoes. this yielded diagnostic fragments of 169, 362, 265 and 426 base pairs for dengue virus types 1, 2, 3 and 4, respectively. from 1997 to 2000, 54 (6.9%) of 781 aedes aegypti and 67 (2.9%) ... | 2002 | 11952948 |
| the 2005 dengue epidemic in singapore: epidemiology, prevention and control. | we investigated the 2005 outbreak of dengue fever (df)/dengue haemorrhagic fever (dhf) to determine its epidemiological, virological and entomological features to further understand the unprecedented resurgence. | 2008 | 18695764 |