Publications

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is900 pcr to detect mycobacterium paratuberculosis in retail supplies of whole pasteurized cows' milk in england and wales.is900 pcr for mycobacterium paratuberculosis was applied to cream, whey, and pellet fractions of centrifuged whole cows' milk. the test and simultaneous control reactions gave correct results for spiked milk and for native milk samples obtained directly from m. paratuberculosis-free, subclinically infected, and clinically infected cows. the test was then applied to units of whole pasteurized cows' milk widely obtained from retail outlets throughout central and southern england from september 199 ...19968795236
assessing the impact of tailored biosecurity advice on farmer behaviour and pathogen presence in beef herds in england and wales.the term 'biosecurity' encompasses many measures farmers can take to reduce the risk of pathogen incursion or spread. as the best strategy will vary between settings, veterinarians play an important role in assessing risk and providing advice, but effectiveness requires farmer acceptance and implementation. the aim of this study was to assess the effectiveness of specifically-tailored biosecurity advice packages in reducing endemic pathogen presence on uk beef suckler farms. one hundred and sixt ...201627931934
crohn's disease caused by mycobacterium avium subspecies paratuberculosis: a public health tragedy whose resolution is long overdue. 200211800469
control of johne's disease in a flock of sheep by vaccination. 19938236728
seroprevalence and epidemiological characteristics of mycobacterium avium subsp. paratuberculosis on 114 cattle farms in south west england.between december 2002 and april 2006, 114 cattle farms in the south west of england were visited at least once, with 100 farms visited three times. a total of 29,782 serum samples were collected from 15,736 individually identified cattle. the sera were tested for the presence of antibodies against mycobacterium avium subsp. paratuberculosis (map) using an indirect enzyme-linked immunosorbent assay (elisa). the mean seroprevalence in herds sampled three times was 7.1%; 10.1% of cattle had at leas ...200919327856
an abattoir-based study of the prevalence of subclinical johne's disease in adult cattle in south west england.the prevalance of subclinical johne's disease was estimated in adult cattle slaughtered at three major abattoirs in south west england. a polymerase chain reaction (pcr) based on is900 was used to detect mycobacterium paratuberculosis in intestinal lymph nodes of 1553 cattle. culture was also carried out on all pcr positive and inconclusive samples. the prevalence of subclinical disease in adult cattle was 3.5% (95% confidence intervals (ci) 2.6-4.7) by pcr and 2.6% (ci 1.8-3.6) by culture. the ...19968666083
incidence of mycobacterium paratuberculosis in raw sheep and goats' milk in england, wales and northern ireland.a blind survey of 104 raw sheep and goats' milk samples (90 goat, 14 sheeps) from bulk tanks on farms throughout england, wales and northern ireland was carried out over a 5-month period (january-may 1998) in order to determine the incidence of mycobacterium paratuberculosis. each milk sample (100 ml) was divided into two 50ml portions. one portion was decontaminated with 0.75% hexadecylpyridinium chloride for 5h before culture on slopes of herrold's egg yolk medium and in bactec radiometric med ...200111230934
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