Publications

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outbreaks of swine vesicular disease in japan: virus isolation and epizootiological survey.outbreaks of a vesicular disease occurred among pigs in kanagawa and ibaraki prefectures in japan in november, 1973. another outbreak was observed in aichi prefecture in december. the clinical signs of the disease observed included fever and vesicular lesions on the coronary bands, bulbs of the heel and in the interdigital spaces. in some pigs, vesicular lesions were observed on the snout, tongue and skin overlying the legs and abdomen. all the vesicular samples produced cytopathic changes on cu ...1975174009
a single-tube multiplex pcr for rapid detection in feces of 10 viruses causing diarrhea.a novel multiplex polymerase chain reaction assay was developed to identify 10 viruses in a single tube. the assay was targeted to detect group a and c rotaviruses, adenovirus, norovirus gi, norovirus gii, sapovirus, astrovirus, aichi virus, parechovirus, and enterovirus. a total of 235 stool samples were collected from infants and children with acute gastroenteritis in kyoto, japan, from 2008 to 2009, then tested by this novel multiplex pcr and compared with a multiplex pcr described previously ...201121349292
prevalence of coxsackievirus a5, a6, and a10 in patients with herpangina in aichi prefecture, 2005. 200516377876
isolation and identification of a novel human parechovirus.a cytopathic agent (a308/99) was isolated using vero cells from a stool specimen of a 1-year-old patient with transient paralysis. the agent was approximately 28 nm in diameter with a distinct ultrastructure resembling the virus particle of an enterovirus. it could not be neutralized by antisera against human picornaviruses such as human enterovirus, aichi virus or human parechovirus. the virion contained three capsid proteins with molecular masses of 38, 30.3 and 30 kda. determination of the co ...200414769896
prevalence of newly isolated, cytopathic small round virus (aichi strain) in japan.cytopathic small round virus (aichi strain), isolated from a patient with oyster-associated gastroenteritis, showed no reaction in the polymerase chain reaction method for enteroviruses or in the enzyme-linked immunosorbent assay (elisa) for the five serotypes of astroviruses. our elisa was sensitive in detecting the aichi strain antigen in stool samples, but there was no reaction in this elisa with any non-aichi strains of enteric viruses, with such origins as enterovirus, rotavirus, norwalk vi ...19938263178
[the relationship of clinical manifestations and age distribution in enterovirus infections--viral isolation and seroepidemiology in aichi prefecture].we studied the epidemiology of enterovirus infection in aichi prefecture from 1985 to 1989. we examined the age distribution of aseptic meningitis patients (am) and exanthematous disease patients (ex) and a seroepidemiological study of echovirus type 7 (e7), e9, e18 and group a coxsackievirus type 9 (ca9), was performed. the results is as follows: 1) e7, e9, e18 and ca9 were isolated from am and ex but e6, e11 and group b coxsackie viruses (cb) were isolated in fewer cases from ex. 2) the am was ...19921338208
a 2-year survey of the prevalence of enteric viral infections in children compared with contamination in locally-harvested oysters.we studied, for two years, the prevalence of indigenous human enteric viruses in wild oysters gathered each month from the bottom of mikawa bay, aichi prefecture, japan. viruses were detected periodically in 9 out of 54 oyster pools prepared by the acid or polyethylene glycol precipitation method although all these 9 pools met current national bacteriological safety standards. since most of the serotypes of the enteric viruses detected in the oysters were identical with those of viruses isolated ...19921312478
a novel rt-multiplex pcr for detection of aichi virus, human parechovirus, enteroviruses, and human bocavirus among infants and children with acute gastroenteritis.a novel reverse transcription-multiplex polymerase chain reaction assay was developed to detect aichi virus, human parechovirus, enteroviruses, and human bocavirus. a mixture of four pairs of published specific primers, 6261 and 6779, ev22(+) and ev22(-), f1 and r1, 188f and 542r, was used to amplify the viral genomes and specifically generate four different amplicon sizes of 519, 270, 440, and 354 bp for aichi virus, human parechovirus, enteroviruses, and human bocavirus, respectively. a total ...201020691209
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