Publications

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[an epidemic of hand-foot-and-mouth disease due to coxsackie a16 in tianjin city]. 19852990718
rapid quantification of infectious enterovirus from surface water in bohai bay, china using an integrated cell culture-qpcr assay.to rapidly quantify infectious enteroviruses polluting the coastal seawaters, a newly developed integrated cell culture and reverse transcription quantitative pcr (icc-rt-qpcr) assay was used to identify the contamination by enteroviruses in winter seawater samples of bohai bay, tianjin, china. the gene copies of enteroviral 5'utrs correlated to the initial inoculum numbers across the concentration range of 0.05-500 pfu ml(-1) (correlation coefficient (r(2)) was 0.9667). icc-qpcr revealed that f ...201121889173
detection and quantification of enteroviruses in coastal seawaters from bohai bay, tianjin, china.an 8-month survey was conducted to detect and quantify enteroviruses in tianjin coastal seawaters of bohai bay to assess coastal water quality. ten water samples were collected from bohai bay for the detection and quantification of enteroviruses by conventional reverse transcription polymerase chain reaction (rt-pcr) and sybr green real-time quantitative rt-pcr (qrt-pcr). total viral nucleic acid was extracted from 500 ml of seawater samples concentrated by centricon plus-70 centrifugal filter d ...201020397399
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