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serodiagnosis of babesia gibsoni infection in dogs by an improved enzyme-linked immunosorbent assay with recombinant truncated p50.the surface antigen p50 of babesia gibsoni is an important candidate for the development of a diagnostic reagent for canine piroplasmosis. in order to establish an effective diagnostic method for practical use, the gene encoding truncated p50 (p50t) lacking a signal peptide and c-terminal hydrophobic regions were cloned and expressed in escherichia coli as a fusion protein with glutathione s-transferase (gst). more than 90% portion of the gst-p50t was expressed as a soluble form, in contrast wit ...200415644601
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