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pcr detection of lizard malaria parasites: prevalence of plasmodium infections with low-level parasitemia differs by site and season.plasmodium-specific polymerase chain reaction (pcr) primers allowed detection of infections with very low-level parasitemia for 3 species of malaria parasites infecting anolis lizards at 2 caribbean sites, puerto rico and saba, netherlands antilles. a verification study, using a single-tube nested pcr to eliminate contamination, showed that infections as low as 1 parasite per millions of erythrocytes could be detected by amplifying a 673 bp fragment of the cytochrome b gene. very low-level paras ...200516539045
distribution and abundance of two malarial parasites of the endemic anolis lizard of saba island, netherlands antilles.the ecology of 2 parasites, plasmodium floridense and plasmodium azurophilum, was studied in the endemic lizard, anolis sabanus, on saba island in the eastern caribbean. prevalence of the two parasite species was similar, but prevalence varied among sites. lowest prevalence occurred at dry, windy sites or cool, wet mountain peaks (0-20%); the parasites were more common at most other locations on the island (40-80% infected; overall prevalence = 47%). high and low prevalence sites sometimes were ...19968636844
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