| polymorphism at the merozoite surface protein-3alpha locus of plasmodium vivax: global and local diversity. | allelic diversity at the plasmodium vivax merozoite surface protein-3alpha (pvmsp-3alpha) locus was investigated using a combined polymerase chain reaction/restriction fragment length polymorphism (pcr/rflp) protocol. symptomatic patient isolates from global geographic origins showed a high level of polymorphism at the nucleotide level. these samples were used to validate the sensitivity, specificity, and reproducibility of the pcr/rflp method. it was then used to investigate pvmsp3alpha diversi ... | 1999 | 10548283 |
| isoenzimatic analysis of four anopheles (kerteszia) cruzii (diptera: culicidae) populations of brazil. | anopheles cruzii is a small sylvatic mosquito and primary human plasmodium vector in southern brazil. the distribution of this bromeliad-breeding mosquito follows the atlantic forest coastal distribution, where bromeliads are abundant. morphological, genetic, and molecular polymorphisms among different populations have been reported and it has recently been suggested that an. cruzii is a complex of cryptic species. the aim of this work is to analyze the gene flow between different populations of ... | 2004 | 15543408 |
| antibody response of naturally infected individuals to recombinant plasmodium vivax apical membrane antigen-1. | in the present study, we evaluate the naturally acquired antibody response to the plasmodium vivax apical membrane antigen 1 (pvama-1), a leading vaccine candidate against malaria. the gene encoding the pvama-1 ectodomain region (amino acids 43-487) was cloned by pcr using genomic dna from a brazilian individual with patent p. vivax infection. the predicted amino acid sequence displayed a high degree of identity (97.3%) with a previously published sequence from the p. vivax salvador strain. a re ... | 2004 | 15710439 |
| molecular epidemiology of plasmodium vivax in the state of amazonas, brazil. | over the past 2 decades, the amazon region of brazil has experienced reemergence of plasmodium vivax malaria, with reported occurrence of severe disease. the frequency and manifestations of this severe disease are unlike previous clinical experience. the hypothesis has been raised that the occurrence of severe disease may relate to the emergence of a variant form of the parasite. to test this hypothesis, we conducted a retrospective cohort study of p. vivax strains in the state of amazonas. we d ... | 2007 | 17397788 |
| assessing the molecular divergence between anopheles (kerteszia) cruzii populations from brazil using the timeless gene: further evidence of a species complex. | anopheles (kerteszia) cruzii was the most important vector of human malaria in southern brazil between 1930-1960. nowadays it is still considered an important plasmodium spp. vector in southern and south-eastern brazil, incriminated for oligosymptomatic malaria. previous studies based on the analysis of x chromosome banding patterns and inversion frequencies in an. cruzii populations from these areas have suggested the occurrence of three sibling species. in contrast, two genetically distinct gr ... | 2009 | 19358734 |
| estimation of divergence time between two sibling species of the anopheles (kerteszia) cruzii complex using a multilocus approach. | anopheles cruzii is the primary human plasmodium vector in southern and southeastern brazil. the distribution of this mosquito follows the coast of the brazilian atlantic forest. previous studies indicated that an. cruzii is a complex of cryptic species. | 2010 | 20356389 |