| rapid and reliable serological diagnosis of enteric fever: comparative sensitivity and specificity of typhidot and typhidot-m tests in febrile malaysian children. | the typhidot test, which detects igm and igg antibodies to a salmonella typhi-specific outer membrane protein, is as sensitive as, and more specific than, the widal test in the diagnosis of enteric fever in malaysian children. it is easier and quicker to perform. in order to increase diagnostic accuracy in an area of high endemicity, the typhidot-m test has been developed in which igg is first removed. this theoretically allows improved detection of igm, and thus would differentiate new from rec ... | 1999 | 10206117 |
| longevity of antibody responses to a salmonella typhi-specific outer membrane protein: interpretation of a dot enzyme immunosorbent assay in an area of high typhoid fever endemicity. | the objective of this study was to investigate the longevity of positive dot enzyme immunosorbent assay (dot eia) results for igm and igg to a salmonella typhi outer membrane protein in malaysian children with enteric fever. the patients were children one month to 12 years of age with clinical evidence of typhoid fever, positive blood or stool cultures for s. typhi, and/or a positive widal test result who were admitted over a two-year period to general hospital (kota bharu, malaysia). these pati ... | 1997 | 9430522 |
| retrospective review of dot enzyme immunoassay test for typhoid fever in an endemic area. | typhoid fever remains a common problem in malaysia, but for its diagnosis both blood culture and the widal test have drawbacks. a dot enzyme immunoassay (eia) has been developed which detects igm and igg antibodies to a specific 50 kda outer membrane protein on salmonella typhi. this study was performed among outpatients attending the university hospital in kelantan, a state on the east coast of peninsular malaysia where typhoid is endemic. the dot eia was done on 149 outpatients of all ages in ... | 1995 | 9139364 |
| identification of carriers among individuals recruited in the typhoid registry in malaysia using stool culture, polymerase chain reaction, and dot enzyme immunoassay as detection tools. | chronic carriers of salmonella typhi act as reservoirs for the organism and become the agents of typhoid outbreaks in a community. in this study, chronic carriers in kelantan, malaysia were first identified using the culture and polymerase chain reaction method. then, a novel serological tool, designated typhidot-c, was evaluated in retrospect using the detected individuals as control positives. chronic carriage positive by the culture and polymerase chain reaction method was recorded at 3.6% (4 ... | 2015 | 23000800 |
| rapid detection of salmonella typhi by loop-mediated isothermal amplification (lamp) method. | an in-house loop-mediated isothermal amplification (lamp) reaction was established and evaluated for sensitivity and specificity in detecting the presence of salmonella typhi (s. typhi) isolates from kelantan, malaysia. three sets of primers consisting of two outer and 4 inner were designed based on locus stbhuccb_38510 of chaperone papd of s. typhi genes. the reaction was optimised using genomic dna of s. typhi atcc7251 as the template. the products were visualised directly by colour changes of ... | 2014 | 25763045 |
| genetic fine structure of a salmonella enterica serovar typhi strain associated with the 2005 outbreak of typhoid fever in kelantan, malaysia. | among enteric pathogens, salmonella enterica serovar typhi is responsible for the largest number of food-borne outbreaks and fatalities. the ability of the pathogen to cause systemic infection for extended durations leads to a high cost of disease control. chronic carriers play important roles in the evolution of salmonella typhi; therefore, identification and in-depth characterization of isolates from clinical cases and carriers, especially those from zones of endemicity where the pathogen has ... | 2012 | 22689247 |
| genome sequence and comparative pathogenomics analysis of a salmonella enterica serovar typhi strain associated with a typhoid carrier in malaysia. | salmonella enterica serovar typhi is a human pathogen that causes typhoid fever predominantly in developing countries. in this article, we describe the whole genome sequence of the s. typhi strain cr0044 isolated from a typhoid fever carrier in kelantan, malaysia. these data will further enhance the understanding of its host persistence and adaptive mechanism. | 2012 | 23045488 |