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isolation and characterization of sporothrix schenckii from clinical and environmental sources associated with the largest u.s. epidemic of sporotrichosis.the largest recorded epidemic of sporotrichosis in the united states occurred in 1988 and involved a total of 84 cases in 15 states. all cases were associated with wisconsin-grown sphagnum moss. twenty-one clinical isolates of sporothrix schenckii and 69 environmental isolates of sporothrix spp. from the epidemic were characterized and compared. the environmental isolates were recovered from 102 samples of sphagnum moss and other material by using direct plating techniques. characteristics exami ...19911864926
a charmed life.boris magasanik was born in kharkoff, ukraine, on december 19, 1919. he received his preliminary and secondary education in vienna, austria, and studied chemistry at the university of vienna in 1937. he continued his studies at city college, new york (bs, 1941) and after one semester of graduate study at pennsylvania state university, served in the us army in england and france from 1942-1945. he obtained his phd degree in biochemistry from columbia university in 1948. a faculty member in the de ...19947826001
lung parasites of shrews from pennsylvania.we examined lung parasites of three species of soricids, sorex cinereus (n = 58), sorex fumeus (n = 23) and blarina brevicauda (n = 45) collected from pennsylvania (usa), from 1990 to 1995. yeast-like cells of hisfoplasma capsulatum var. capsulatum were found in lung sections stained with grocott's modification of gomori's methenamine silver, periodic acid-schiff, giemsa, and hematoxylin-eosin in two (3%) s. cinereus, eight (35%) s. fumeus and two (4%) b. brevicauda. the number of spores of h. c ...19979131560
spectrum of fungal keratitis at wills eye hospital, philadelphia, pennsylvania.to report the spectrum of fungal keratitis at wills eye hospital, philadelphia.200010832689
outbreaks of clinical mastitis caused by trichosporon beigelii in dairy herds.trichosporon beigelii is widely distributed in nature and is classically associated with white piedra, a mycosis that may involve the hair of the human body. intramammary infections caused by t beigelii may be fatal in cows; the prevalence in affected dairy herds may be high. affected cows may have hyperthermia, swelling of the udder, and substantially decreased milk production or agalactia. intramammary infections caused by yeast, including t beigelii, may also be associated with high bacterial ...200111195831
retrospective study: the presence of malassezia in feline skin biopsies. a clinicopathological study.malassezia spp. dermatitis, a rare disorder in cats, has previously been associated with immune suppression and internal malignancies. this study evaluates the presence and importance of malassezia spp. in feline biopsy specimens submitted for histopathological examination. five hundred and fifty haematoxylin and eosin-stained skin biopsy specimens received for histopathological examination between january 1999 and november 2000 were reviewed. fifteen (2.7%) submissions contained malassezia orga ...200211896965
saccharomyces cerevisiae and saccharomyces paradoxus coexist in a natural woodland site in north america and display different levels of reproductive isolation from european conspecifics.we report the isolation of multiple strains of saccharomyces cerevisiae and saccharomyces paradoxus from a natural woodland site in southeastern pennsylvania, usa, using enrichment culturing in a medium containing 7.6% (v/v) ethanol. the method was applied to bark and flux material collected from broad-leaved trees (mostly quercus spp.) and to associated soils. many candidate wild strains of saccharomyces were isolated using this method, most of them from soils associated with oaks. matings to g ...200212702333
predictive value of the clinical diagnosis of lower genital tract infection in women.we hypothesized that diagnostic approaches to lower genital tract infections are inaccurate and proposed this study to evaluate typical approaches.200415118630
microbiological and molecular determination of mycobiota in fresh and ensiled maize silage.the mycobiota of fresh and ensiled maize was studied with culturing techniques and a dna sequence-based approach. freshly chopped and ensiled maize were collected for 2 y from 12 farms in pennsylvania. samples were plated on selective media and isolates identified by morphology and sequences of the internal transcribed spacer regions of rdna, 800-900 bp of the 5' end of the translation elongation factor 1-alpha gene and a portion of the roda gene (aspergillus fumigatus only). its regions were am ...200717682779
a yeast estrogen screen without extraction provides fast, reliable measures of estrogenic activity.yeast estrogen screen (yes) has been used since 1996 as a bioassay to quantify activity in wastewater. here we present a modification of yes to measure estrogenic activity in water. this modification, called yeast estrogen screen no extraction (yesne), is faster and easier than the commonly used method. the modified method can detect 17ß-estradiol equivalent concentrations down to 1.1ng/l. the median effective concentration value (ec50) is 1.2e to 10. it detected average influent concentrations ...201121755530
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