| recombinations in individuals homozygous by descent localize the friedreich ataxia locus in a cloned 450-kb interval. | the locus for friedreich ataxia (frda), a severe neurodegenerative disease, is tightly linked to markers d9s5 and d9s15, and analysis of rare recombination events has suggested the order cen-frda-d9s5-d9s15-qter. we report here the construction of a yac contig extending 800 kb centromeric to d9s5 and the isolation of five new microsatellite markers from this region. in order to map these markers with respect to the frda locus, all within a 1-cm confidence interval, we sought to increase the gene ... | 1994 | 8198128 |
| high frequency of p53 mutations in human oral epithelial dysplasia and primary squamous cell carcinoma detected by yeast functional assay. | to determine the timing and actual incidence of p53 mutations in oral epithelial lesions, we examined 33 primary squamous cell carcinomas (sccs), 14 dysplasias and six hyperplasias from japanese patients by a combination of yeast functional assay and dna sequencing. the assay detects mutations of p53 mrna between codons 67 and 347 on the basis of the dna-binding activity of the protein. twenty-six sccs (79%) and five dysplasias (36%) were positive for p53 mutation, while all six hyperplasias wer ... | 1997 | 9400993 |
| a new triterpene saponin from pittosporum viridiflorum from the madagascar rainforest. | a novel triterpenoid saponin, pittoviridoside (1), which possesses an unusual 2,3,4-trisubstituted glycosidic linkage, has been isolated from pittosporum viridiflorum using the engineered yeast strains 1138, 1140, 1353, and sc-7 for bioactivity-guided fractionation. the structure of this compound was determined to be 3-o-[beta-d-glucopyranosyl(1-->2)]-[alpha-d-arabinopyranosyl(1-->3)],[alpha-l-arabinofuranosyl(1-->4)-beta-d-glucuronopyranosyl-21-angeloyl-22-senecioylolean-12-en-3beta,15alpha,16a ... | 2002 | 11809069 |
| point mutations in the dihydrofolate reductase and dihydropteroate synthase genes of plasmodium falciparum and resistance to sulfadoxine-pyrimethamine in sri lanka. | sulfadoxine-pyrimethamine (sp) is the second-line treatment for plasmodium falciparum malaria in sri lanka. resistance to sp is caused by point mutations in the dihydrofolate reductase (pf-dhfr) and dihydropteroate synthase (pf-dhps) genes of p. falciparum. we determined the genotype of pf-dhfr and pf-dhps and the clinical response to sp in 30 field isolates of p. falciparum from sri lanka. all patients treated with sp had an adequate clinical response. eighty-five percent (23 of 27) of pure fie ... | 2006 | 16474070 |
| oral submucous fibrosis and oral yeast carriage - a case control study in sri lankan patients. | oral submucous fibrosis (osmf) is a well-known precancerous condition. epithelial atrophy is one of the key features in osmf. presence of candida in the mouth together with epithelial changes may predispose to candidal infection. candidal infection together with other co-factors may also induce epithelial atypia and dysplasia leading to malignant change. the purpose of this study was to determine the prevalence of oral yeast carriage in patients with osmf and to compare the carriage with the nor ... | 2007 | 17305774 |
| epidemiological phenotypic characteristics of vaginal yeasts at the comoros. | the aim of the prospective, descriptive survey conducted in moroni, comoros was to establish the distribution of vulvovaginal mycoses in pregnant and symptomatic women and to study the epidemiological characteristics of the yeast isolates. all isolated strains were epidemiologically evaluated by phenotypical methods. three phenotypic epidemiological studies were performed by morphotyping methods (including the colour reaction according to quindòs et al. and structural morphotypes as described by ... | 2009 | 18983428 |