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long-term cryptosporidium typing reveals the aetiology and species-specific epidemiology of human cryptosporidiosis in england and wales, 2000 to 2003.to improve understanding of the aetiology and epidemiology of human cryptosporidiosis, over 8,000 cryptosporidium isolates were submitted for typing to the species level over a four year period. the majority were either cryptosporidium parvum (45.9%) or cryptosporidium hominis (49.2%). dual infection occurred in 40 (0.5%) cases and six other known cryptosporidium species or genotypes were found in 67 (0.9%) cases. these were cryptosporidium meleagridis, cryptosporidium felis, cryptosporidium can ...200919161717
detection of cryptosporidium spp. from human faeces by pcr-rflp, cloning and sequencing.in this study, we compared and validated a nested abc polymerase chain reaction restriction fragment length polymorphism (pcr-rflp) assay to amplify cryptosporidium parvum oocyst wall protein (cowp) gene fragment with a previous nested pcr-rflp method. when rflp of cowp gene did not provide clear results, we further analysed samples by cloning and sequencing for species and genotype identification. abc-pcr was performed on dna extracted from human faecal samples collected in england where crypto ...200918979120
genetic analysis of cryptosporidium from 2414 humans with diarrhoea in england between 1985 and 2000.the characterization of cryptosporidium using dna extracted from whole faecal samples collected from 2414 humans with diarrhoea in england between 1985 and 2000 where cryptosporidial oocysts were detected using conventional methods is described. characterization was achieved by pcr/rflp and dna sequencing of fragments of the cryptosporidium oocyst wall protein and the 18s rdna genes. cryptosporidium parvum was detected in 56.1 % of cases, cryptosporidium hominis in 41.7 % and a mixture of c. par ...200616687587
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