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molecular epidemiology of chicken anemia virus (cav) in southeastern chinese live birds markets.between january 2004 and december 2005, cloacal swabs from essentially healthy chickens and silky chickens from live birds markets in guangdong and hunan provinces in southeastern china were screened for chicken anemia virus (cav) by polymerase chain reaction. phylogenetic analysis of the major structural protein vp1 sequences showed no clear genotype cluster and no correlation with the geographic origin of cav strains. virus evolution at the amino acid level was very slow, which corresponds to ...200818459299
molecular characterization of h9n2 influenza virus isolated from mink and its pathogenesis in mink.in mid-august 2013, two h9n2 influenza viruses, named a/mink/shandong/f6/2013 (mk/sd/f6/13) and a/mink/shandong/f10/2013 (mk/sd/f10/13), were isolated from lung samples of 2 of 45 farmed mink exhibiting respiratory signs in mideastern shandong province, china. the seroprevalence of antibodies to h9n2 in mink was 20% (53/265). based on sequence analysis, the eight nucleotide sequences showed 99.7-100% identity between mk/sd/f6/13 and mk/sd/f10/13. the ha, np and ns genes of mk/sd/f6/13 and mk/sd/ ...201525655813
[complete genome phylogenetic analysis of five h9n2 avian influenza viruses isolated from poultry flocks in qinghai lake region].five h9n2 avian influenza virus strains were isolated from the environmental samples in live poultry market in qinghai lake region from july to september, 2012. to evaluate the phylogenetic characteristics of these h9n2 isolates, the eight gene segments were amplified by rt-pcr and sequenced. the phylogenetic and molecular characteristics of the five strains were analyzed. the results showed that the ha genes of five strains shared 93. 2%-99. 1% nucleotide identities with each other, and the na ...201424923162
[the first confirmed human case of avian influenza a (h5n1) in mainland, china].to ascertain the causation of a family cluster involving two undefined pneumonia cases, a 12-year-old girl and her brother, reported october, 2005 in xiangtan county, hunan province.200616875527
characterization of h5n1 influenza a viruses isolated from domestic green-winged teal.two avian influenza virus strains, a/domestic green-winged teal/hunan/67/2005 (h5n1) (d-gwt/67) and a/domestic green-winged teal/hunan/79/2005 (h5n1) (d-gwt/79), were isolated from healthy domestic green-winged teals (anas crecca) in hunan province, south china. genomic analysis showed that both isolates were reassortants. the hemagglutinin (ha) genes of the two isolates were closely related to that of an h5n1 strain isolated from tree sparrow (a/tree sparrow/henan/1/04). the neuraminidase (na) ...200918825495
fowl adenovirus species c serotype 4 is attributed to the emergence of hepatitis-hydropericardium syndrome in chickens in china.since july in 2015, an emerging infectious disease of hepatitis-hydropericardium syndrome (hhs) was prevalent in chicken flocks in china. to confirm the causative agent and investigate the epidemiology of the disease, a total of 38 chicken flocks including 187 samples from jilin, liaoning, heilongjiang, henan, anhui, hubei, jiangxi, xinjiang, shandong and hunan provinces in china were collected and determined by pcr detection, sequencing, phylogenetic analysis and virus isolation. 81 samples (po ...201627615553
temporal and spatial characteristics of highly pathogenic avian influenza outbreaks in china during 2004 to 2015.identifying the temporal and spatial characteristics of highly pathogenic avian influenza (hpai) outbreaks is very important for developing effective and appropriate countermeasures against hpai and promoting sustainable development in the poultry industry. this study aimed to analyze four aspects of the temporal and spatial characteristics of hpai outbreaks in china, including the frequency of hpai outbreaks, numbers of dead animals (died or culled), types of hpai viruses, and species of infect ...201728453859
excretion of manure-borne estrogens and androgens and their potential risk estimation in the yangtze river basin.the yangtze river is the longest river in china, and the river basin spans one fifth of the area of the whole country. based on statistical data, the excretion of manure-borne steroid hormones, including steroid estrogens (ses) and steroid androgens (sas), in 10 provinces of china within the region has been estimated. the potential environmental and ecological risk of manure-borne steroid estrogens to the surface water in this region was also assessed. the manure-borne se and sa excretions in th ...201526574094
genotype diversity of h9n2 viruses isolated from wild birds and chickens in hunan province, china.three h9n2 avian influenza viruses were isolated from the dongting lake wetland, among which one was from fresh egret feces, the other two were from chicken cloacal swabs in poultry markets. phylogenetic analyses suggested that eight genes of the egret-derived h9n2 virus might come from korean-like or american-like lineages. the two poultry-derived h9n2 viruses were reassortants between the ck/bj/94-like and g1-like viruses. except the pb1 genes (90.6%), the nucleotide sequence of other internal ...201424979703
emerging multiple reassortant h5n5 avian influenza viruses in ducks, china, 2008.three highly pathogenic h5n5 avian influenza viruses (hpai), a/duck/guangdong/wy11/2008 (wy11), a/duck/guangdong/wy19/2008 (wy19), and a/duck/guangdong/wy24/2008 (wy24) were isolated from ducks in southern china in april 2008. here, we characterized these viruses by performing sequencing and phylogenetic analyses of their viral genes, assessing their virulence in ducks and mice, and performing cross-protection experiments in chickens. sequence analysis revealed that the ha genes of these h5n5 vi ...201324080350
characterisation of a highly pathogenic h5n1 clade 2.3.2 influenza virus isolated from swans in shanghai, china.in spring 2009, one strain of h5n1 clade 2.3.2 virus was isolated from wild swans in shanghai, indicating the importance of the wild swan in the ecology of this highly pathogenic avian influenza virus (hpaiv) in eastern china. pathogenicity experiments conducted in this study indicated that the virus was highly pathogenic for chickens but lowly pathogenic for mammalian hosts, as evidenced by reduced infection of mice. the analysis of complete genome sequences and genetic evolution showed that a/ ...201121904849
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