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identification and genome characterization of the first sicinivirus isolate from chickens in mainland china by using viral metagenomics.unlike traditional virus isolation and sequencing approaches, sequence-independent amplification based viral metagenomics technique allows one to discover unexpected or novel viruses efficiently while bypassing culturing step. here we report the discovery of the first sicinivirus isolate (designated as strain jsy) of picornaviruses from commercial layer chickens in mainland china by using a viral metagenomics technique. this sicinivirus isolate, which contains a whole genome of 9,797 nucleotides ...201526461027
prevalence of low pathogenic avian influenza virus in one live bird market in eastern china from 2002-2011.the role of live bird markets (lbms) in the perpetuation of avian influenza virus (aiv) has been well studied worldwide; however, there is a paucity of information on the prevalence of different aiv subtypes in lbms in eastern china. in this study, long-term surveillance was conducted to investigate the prevalence of aiv in chickens, ducks, and geese in an lbm in yangzhou city, jiangsu province, eastern china, between july 2002 and may 2011. the study used primary virus isolation and subtype-spe ...201323678747
occurrence and reassortment of avian influenza a (h7n9) viruses derived from coinfected birds in china.over the course of two waves of infection, h7n9 avian influenza a virus has caused 436 human infections and claimed 170 lives in china as of july 2014. to investigate the prevalence and genetic diversity of h7n9, we surveyed avian influenza viruses in poultry in jiangsu province within the outbreak epicenter. we found frequent occurrence of h7n9/h9n2 coinfection in chickens. molecular clock phylogenetic analysis confirms coinfection by h7n9/h9n2 viruses and also reveals that the identity of the ...201425210174
pollution characteristics of 23 veterinary antibiotics in livestock manure and manure-amended soils in jiangsu province, china.the aim of this study was to investigate the pollution characteristics of typical veterinary antibiotics in manure and soil of livestock farms in jiangsu province. this investigation employed solid-phase extraction (spe) coupled with ultra-performance liquid chromatography-tandem mass spectrometry (uplc-ms/ms). a total of 53 manure and 50 amended soil samples from 16 livestock farms in jiangsu province were collected for analysis. in the manure samples, the highest detected frequencies and conce ...201626963628
characterization of a highly pathogenic avian influenza h5n1 clade 2.3.4 virus isolated from a tree sparrow.the spread of h5n1 virus into a wide range of avian and mammalian species may facilitate the adaptation of the virus to human populations. in the present study, a survey of avian influenza virus in tree sparrows was performed and a hpai h5n1 virus (a/tree sparrow/jiangsu/1/08) was isolated. the h5n1 virus was found to be a genotype v variant belonging to clade 2.3.4, which had newly emerged in southern china in 2005. genetic analysis showed that it had a close relation with a/jiangsu/1/07 (h5n1) ...201019804800
[biological characteristics and sequence analysis of fusion genes of newcastle disease virus isolates].twenty newcastle disease virus (ndv) strains were isolated from chickens and geese in the field outbreaks during 2005 and 2006 in some regions of jiangsu and guangxi province. assessment of the virulence by mdt and icpi, rt-pcr and sequence analysis of fusion protein gene were used to compare the properties of ndv isolates. the results indicated that mdt and icpi of the isolates were 45.3h - 58.2h and 1.61 - 2.00 respectively, which confirmed that the all ndv isolates were highly virulent. and t ...200919678566
serologic evidence of prevalent avian h3 subtype influenza virus infection in chickens.h3-subtype influenza viruses are known to infect avian and mammalian species, including humans. however, little is known about the prevalence of h3 influenza virus infection in chicken populations in china. therefore, a serologic survey of chickens was conducted in china to investigate the seroprevalence of avian h3-subtype influenza virus. anti-h3 antibodies were assayed by using hemagglutination inhibition (hi) and confirmatory virus neutralization (vn) testing of 4598 serum samples, collected ...200919630224
[surveillance of escherichia coli o157:h7 among animals in jiangsu province in 1999].to understand the escherichia coli o157:h7 carrier rate of host animals and the toxic gene of the strains in different areas in jiangsu province.200212015089
[a serological survey of legionnaires' disease in domestic fowls and animals in chengdu area].a serological survey of antibodies against legionella pneumophila (lp) serotypes in chengdu area by microagglutination test showed that there were high levels of antibodies against serotypes lp1 and lp6 in healthy domestic fowls and animals (rabbits, pigs, chickens, ducks and geese). in rabbits, antibodies against serotypes lp1-lp6 were determined, showing positive rates (titer greater than or equal to 1:16) ranging 6.3-23.8%. among them only the serotype lp3 did not show any positive one. the h ...19892630424
seroprevalence of antibodies against sfts virus infection in farmers and animals, jiangsu, china.severe fever with thrombocytopenia syndrome (sfts) is a newly identified viral zoonosis caused by a phlebovirus. most reported sfts cases are farmers living in rural areas. the seroprevalence of sfts virus in farmers has not been investigated. the current knowledge of sfts virus seroprevalence in animals, especially in wild animals, is still poor.201424793967
molecular characterization of pigeon torque teno virus (pttv) in jiangsu province.the torque teno virus (ttv) is a recently discovered dna virus that has been detected in many different hosts, including humans, livestock and poultry. to date, there is no report of pigeon ttv (pttv) from anywhere in the world. to investigate the distribution of pttv in pigeons from the eastern chinese province of jiangsu and characterize their genomes, we employed pcr to detect pttv in 144 samples collected from 6 pigeon plants in jiangsu province, amplify complete genomes from representative ...201728527409
understanding the recurrent large-scale green tide in the yellow sea: temporal and spatial correlations between multiple geographical, aquacultural and biological factors.the coast of jiangsu province in china - where ulva prolifera has always been firstly spotted before developing into green tides - is uniquely characterized by a huge intertidal radial mudflat. results showed that: (1) propagules of u. prolifera have been consistently present in seawater and sediments of this mudflat and varied with locations and seasons; (2) over 50,000 tons of fermented chicken manure have been applied annually from march to may in coastal animal aquaculture ponds and thereaft ...201323176870
sulfonamide-resistant bacteria and their resistance genes in soils fertilized with manures from jiangsu province, southeastern china.antibiotic-resistant bacteria and genes are recognized as new environmental pollutants that warrant special concern. there were few reports on veterinary antibiotic-resistant bacteria and genes in china. this work systematically analyzed the prevalence and distribution of sulfonamide resistance genes in soils from the environments around poultry and livestock farms in jiangsu province, southeastern china. the results showed that the animal manure application made the spread and abundance of anti ...201425405870
residues of organochlorine pesticides and polycyclic aromatic hydrocarbons in farm-raised livestock feeds and manures in jiangsu, china.the residual levels of 8 organochlorine pesticides (ocps) and 15 priority polycyclic aromatic hydrocarbons (pahs) were determined in pig, chicken, and cow feed and manure samples collected from feedlots in jiangsu province, china. the mean residuals of ocps ranged from 25.35 to 65.62 ng g(-1) in feeds and from 33.46 to 90.89 ng g(-1) in manures. among 4 hexachlorocyclohexanes (hchs), α-hch was the most abundant compound, with a high occurrence above 80% in all kinds of animal feeds and manures. ...201323058310
a new piece in the puzzle of the novel avian-origin influenza a (h7n9) virus.using phylogenetic analysis on newly available sequences, we characterize a/chicken/jiangsu/rd5/2013(h10n9) as currently closest precursor strain for the na segment in the novel avian-origin h7n9 virus responsible for an outbreak in china. we also show that the internal segments of this precursor strain are closely related to those of the presumed precursor for the ha segment, a/duck/zhejiang/12/2011(h7n3), which indicates that the sources of both ha and na donors for the reassortant virus are o ...201324160334
full-length genome sequencing analysis of avian infectious bronchitis virus isolate associated with nephropathogenic infection.infectious bronchitis virus (ibv) produces infectious bronchitis (ib) disease in poultry worldwide. in spite of proper vaccinations against the ibv, new ibv strains are continually emerging worldwide. in this study, a new highly virulent nephropathogenic ibv strain named ck/ch/xdc-2/2013 was identified from a vaccinated flock with clinical signs of ib in the jiangsu province of china. the full-length genome sequence of the isolate was 27,714 nucleotides long, and the genome was organized similar ...201627566728
characterization of antimicrobial resistance and extended-spectrum β-lactamase genes in escherichia coli isolated from chickens.extended-spectrum β-lactamase (esbl)-producing escherichia coli have been frequently isolated from food-producing animals and pose a serious threat to human health. this study collected 195 esbl-producing e. coli isolates from 20 chicken farms and 3 live-bird markets located in northeast china (heilongjiang, liaoning, jilin) and jiangsu province from february 2011 to october 2013. esbl genes, including blactx-m, blatem, and blashv, were detected and characterized, and the susceptibilities of the ...201525785885
stx2vha is the dominant genotype of shiga toxin-producing escherichia coli o157:h7 isolated from patients and domestic animals in three regions of china.shiga toxin-producing escherichia coli(stec) o157: h7 strains were isolated from domestic animals and patients from xuzhou city, jiangsu province, china and the bordering anhui and henan provinces and were examined for the stx genotype. of 390 strains, 277 were identified as genotype stx2vha ; 41, stx2 ; 51, stx2-stx1 ; 1, stx2-stx2vha-stx1 ; 5, stx2-stx2vha ; and 15 were un-typeable. of the 277 stx2vha-bearing isolates, 116 were isolated from goats; 42, cattle; 38, hens, and 35 from pigs. the s ...200516365526
antimicrobial resistance in clinical escherichia coli isolates from poultry and livestock, china.poultry and livestock are the most important reservoirs for pathogenic escherichia coli and use of antimicrobials in animal farming is considered the most important factor promoting the emergence, selection and dissemination of antimicrobial-resistant microorganisms. the aim of our study was to investigate antimicrobial resistance in e. coli isolated from food animals in jiangsu, china. the disc diffusion method was used to determine susceptibility to 18 antimicrobial agents in 862 clinical isol ...201728934348
prevalence of antibiotic resistance genes of wastewater and surface water in livestock farms of jiangsu province, china.the overuse of antibiotics in livestock farms is general, leading to a wide distribution of antibiotic resistance genes (args) in aquatic environment adjacent to livestock farms. however, researches of the distribution and types of args in aquatic environment of china are still in the initial stage. in this study, wastewater and surface water samples were collected from 12 livestock farms (four pig farms, four cattle farms, and four chicken farms) in jiangsu province of china. the prevalence, ab ...201525948386
excretion of manure-borne estrogens and androgens and their potential risk estimation in the yangtze river basin.the yangtze river is the longest river in china, and the river basin spans one fifth of the area of the whole country. based on statistical data, the excretion of manure-borne steroid hormones, including steroid estrogens (ses) and steroid androgens (sas), in 10 provinces of china within the region has been estimated. the potential environmental and ecological risk of manure-borne steroid estrogens to the surface water in this region was also assessed. the manure-borne se and sa excretions in th ...201526574094
temporal and spatial characteristics of highly pathogenic avian influenza outbreaks in china during 2004 to 2015.identifying the temporal and spatial characteristics of highly pathogenic avian influenza (hpai) outbreaks is very important for developing effective and appropriate countermeasures against hpai and promoting sustainable development in the poultry industry. this study aimed to analyze four aspects of the temporal and spatial characteristics of hpai outbreaks in china, including the frequency of hpai outbreaks, numbers of dead animals (died or culled), types of hpai viruses, and species of infect ...201728453859
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