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bacterial diversity in a marine methanol-fed denitrification reactor at the montreal biodome, canada.the bacterial biota of a methanol-fed denitrification reactor used to treat seawater at the montreal biodome were investigated using culture-dependent and molecular biology methods. the microbiota extracted from the reactor carriers were cultivated on three media. three isolate types were recovered and their 16s ribosomal dna (rdna) genes were determined. the analysis showed that the isolate types were related to alpha-proteobacteria. they are members of the hyphomicrobium and paracoccus genera ...200312739074
microbiological community structure of the biofilm of a methanol-fed, marine denitrification system, and identification of the methanol-utilizing microorganisms.we demonstrated in a previous study that the biofilm of the methanol-fed fluidized marine denitrification reactor at the montreal biodome was composed of at least 15 bacterial phylotypes. among those were 16s ribosomal rna (rdna) gene sequences affiliated to hyphomicrobium spp., and methylophaga spp.; the latter made up 70% of a clone library. by using fluorescent in situ hybridization (fish), we investigated the structure of the biofilm during the colonization process in the denitrification rea ...200717394042
functional diversity in the denitrifying biofilm of the methanol-fed marine denitrification system at the montreal biodome.nitrate is a serious problem in closed-circuit public aquariums because its accumulation rapidly becomes toxic to many lifeforms. a moving bed biofilm denitrification reactor was installed at the montreal biodome to treat its 3,250-m(3) seawater system. naturally occurring microorganisms from the seawater affluent colonized the reactor carriers to form a denitrifying biofilm. here, we investigated the functional diversity of this biofilm by retrieving gene sequences related to narg, napa, nirk, ...201122006549
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