descendant of daughter brazilian bcg moreau substrain in this study we assessed the genomic stability of mycobacterium bovis bcg moreau seed lots used in poland for bcg vaccine production since 1955 by pulsed field gel electrophoresis (pfge), amplified fragment length polymorphism (aflp) and random amplification of polymorphic dna (rapd). bcg vaccine lots were more closely related the original lot -m. bovis bcg rio de janeiro moreau compared with seeds used before 1980, which is consistent with seed lot distribution recorded in the archives. we con ...201222749596
bacteriological and molecular detection of mycobacterium bovis in cattle with inconclusive results to intradermal tuberculin tests.a dairy herd (77 cows) from rio de janeiro, brazil, with a history of tuberculosis infection was tested by a comparative cervical test (cct). seventeen cows were reactive and seven were inconclusive (swelling ≥ 2.0 mm and ≤ 3.9 mm, respectively). all of these 24 cows were slaughtered and necropsied ; samples from lungs and lymph nodes were collected for multiplex polymerase chain reaction (pcr) and culturing. infection was confirmed in 23/24 (95.8%) of the slaughtered animals (five by culturing, ...201323010250
a complementary diagnosis of naturally occurring tuberculosis in water buffaloes (bubalus bubalis) in rio de janeiro using a mpb70-elisa, tuberculosis is still a worldwide infection and buffalo breeding represents an important economic activity in various countries, the purpose of this study was to employ an enzyme-linked immunosorbent assay (elisa) using mpb70 as a capture antigen for the diagnosis of naturally occurring tuberculosis in water buffaloes in brazil. after the introduction of newly acquired cattle onto a tuberculosis (tb) free farm, an outbreak of tb was recorded in a mixed herd comprising water buffaloes (21) and ...201223274697
multiple strains of mycobacterium bovis revealed by molecular typing in a herd of cattle.mycobacterium bovis isolates from an outbreak of bovine tuberculosis in a herd of cattle in rio de janeiro, brazil, were analysed by spoligotyping and variable-number tandem repeat pcr analysis of the mycobacterial interspersed repetitive unit and exact tandem repeats. molecular typing revealed a high genetic diversity of strains in the herd. the genetic diversity could be explained by the introduction of infected animals from different sources.201122178356
Identification of Mycobacterium bovis among mycobacterial isolates from human clinical specimens at a university hospital in Rio de Janeiro, Brazil.In 2005 and 2006, 8,121 clinical specimens submitted to the Mycobacteriology Laboratory of the Clementino Fraga Filho University Hospital/Thoracic Diseases Institute, in the city of Rio de Janeiro, Brazil, were inoculated on Löwenstein-Jensen medium containing glycerol and pyruvate. There were 79 mycobacteria isolates that presented growth only on pyruvate-containing medium, and those isolates were selected for the presumptive identification of Mycobacterium bovis. The selected isolates were scr ...201122042400
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