evaluation of enzyme-linked immunosorbent assay using milk samples as a potential screening test of bovine tuberculosis of dairy cows in enzyme-linked immunosorbent assay (elisa) using bulk tank milk samples was evaluated as a screening test for bovine tuberculosis (tb), a contagious chronic disease of cattle. an elisa with mpb70, a major antigen of mycobacterium bovis was performed using paired sets of milk and sera samples from 33 tuberculin-positive and 43 tuberculin-negative cattle. anti-mpb70 antibodies were detected in milk samples and there was a significant correlation between seroreactivities of milk and sera samples ...201020060551
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