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serological profile of toxoplasma gondii and neospora caninum infection in commercial sheep from são paulo state, brazil.neosporosis and toxoplasmosis are two important infections in young and adult sheep, leading to low production and abortion. this study aimed to determine the frequency of antibodies to toxoplasma gondii and neospora caninum in sheep from the eastern region of são paulo state, brazil. serum samples (382) were collected from the sheep and assayed for t. gondii through modified agglutination test (mat) and indirect fluorescent antibody test (ifat), and for n. caninum antibodies, through ifat, with ...201021256676
occurrence and molecular characterization of cryptosporidium spp. isolated from domestic animals in a rural area surrounding atlantic dry forest fragments in teodoro sampaio municipality, state of são paulo, brazil.the aim of this study was to assess the occurrence of cryptosporidium in domestic animals in rural properties surrounding rain forest fragments within the municipality of teodoro sampaio, southeastern brazil. conventional sucrose flotation method followed by molecular characterization of the parasites by sequencing pcr products amplified from ssu rrna gene were used. stool samples were collected from domestic animals raised as pets and livestock in all rural properties surrounding three forest f ...201021184703
genetic diversity among toxoplasma gondii isolates of small ruminants from brazil: novel genotypes revealed.recent studies indicated that toxoplasma gondii isolates of many domestic animal hosts from brazil are genetically and biologically different from those in usa and europe. despite of high pathogenicity of this parasite to small ruminants, the epidemiology and genetic diversity of t. gondii in these animals are not well understood in brazil. in this study, a total of 28 t. gondii samples (16 isolates from sheep in são paulo state, and 12 isolates from goats in the states of são paulo and rio gran ...201020236768
[gastrointestinal nematode parasitism in ewes and lambs raised in são carlos, são paulo].gastrointestinal nematode infections were evaluated in santa inês crossbreed sheep (santa inês predominance) in a rotational grazing system and in lambs kept indoors, born from the breeding of these females with purebred santa ines, dorper and suffolk males. fecal egg counts (fec), fecal cultures, packed cell volume (pcv) and weights were evaluated during two years. climate data were registered. only animals that presented fec higher than 4,000 and/or pcv lower than 21% were drenched with anthel ...200820059831
seroprevalence of and risk factors for toxoplasma gondii in sheep raised in the jaboticabal microregion, são paulo state, brazil.seroprevalence of and risk factors for toxoplasmosis in sheep from different properties in the jaboticabal microregion, são paulo state, brazil were determined. antibodies to toxoplasma gondii were found in sera of 52.0% of 488 sheep tested by indirect fluorescent antibody test (ifat > or =64). t. gondii seropositivity in sheep was significantly associated with gender of the sheep, pasturing system, contact with cats, and the use of mineral supplements and the type of feed.201019589550
shiga toxin-producing escherichia coli and atypical enteropathogenic escherichia coli strains isolated from healthy sheep of different populations in são paulo, brazil.sheep are important carriers of shiga toxin-producing escherichia coli (stec) in several countries. however, there are a few reports about ovine stec in american continent.200919413771
seroprevalence and isolation of toxoplasma gondii from sheep from são paulo state, brazil.sheep are important in the epidemiology of toxoplasma gondii infection but little is known of ovine toxoplasmosis in brazil. antibodies to t. gondii were assayed in sera of 495 sheep from 36 counties of são paulo state, brazil, using the modified agglutination test (mat titer > or =1:25) and found in 120 (24.2%). samples of brain, heart, and diaphragm of 82 seropositive sheep were pooled, digested in pepsin, and bioassayed in mice. toxoplasma gondii was isolated from tissue homogenates of 16 she ...200818576886
[laboratory diagnosis of rabies in the west region of são paulo state].the pólo da alta sorocabana laboratory, presidente prudente, sp, brazil, and the biological institute in são paulo state, performed an evaluation of rabies diagnosis from 1996 to 2003 in the west region of são paulo state. for the tests, the laboratories used direct immunofluorescence and mice inoculation in 4,950 samples, that were sent for analysis involving dogs, cats, cattle, chiroptera (bats) and other animals. according to the results, the laboratories found 74 positive samples; of which 5 ...200616410925
prevalence of anti-toxoplasma gondii and anti-neospora caninum antibodies in ovine from são paulo state, brazil.serum samples from 597 sheep from são paulo state, in the southeastern region of brazil, were tested to determine the prevalence of antibodies directed against toxoplasma gondii (> or = 1:64) and neospora caninum (> or = 1:50) using the indirect fluorescent antibody test (ifat). the animals were divided into three groups based on their age: < or = 1 year, 1-4 years, and > or = 4 years. antibodies to t. gondii were observed in 34.7% of the samples with titers ranging from 64 to 16,384 and igg ant ...200415325042
resistance of santa ines, suffolk and ile de france sheep to naturally acquired gastrointestinal nematode infections.a study was conducted to assess the breed resistance against nematode infections in santa ines, ile de france and suffolk male lambs over a 9-month period in são paulo state, brazil. lambs were born during the winter (year 2000) and were weaned at 2 months of age. the animals were then housed and treated with anthelmintics to eliminate natural infections by gastrointestinal nematodes. in late october 2000, lambs were placed in a paddock, where they stayed until august of the following year. feca ...200415019147
properties of shiga toxin-producing escherichia coli (stec) isolates from sheep in the state of são paulo, brazil.fecal samples from 48 sheep from two farms in são paulo, sp, brazil, were examined to determine the prevalence of shiga toxin-producing escherichia coli (stec). forty-two stec strains were isolated from 25 (52.1%) of 48 sheep feces, and were examined for the presence of genes encoding stec-related virulence factors. twenty-one (50.0%) of the 42 stec isolates were positive for stx(1) and stx(2), 16 isolates (38.1%) were stx(1), and five (11.9%) were stx(2). expression of shiga toxins was demonstr ...200312860080
the detection of toxoplasma gondii by comparing cytology, histopathology, bioassay in mice, and the polymerase chain reaction (pcr).the objective of this study was to compare the different methods of detecting toxoplasma gondii in sheep tissue, tested serologically positive by the indirect immunofluorescent antibody test (ifat). brain, diaphragm, and blood samples were collected from 522 sheep slaughtered at the são manuel abattoir, são paulo state, brazil. brain and diaphragm samples from ifat seropositive animals were digested by both trypsin and pepsin and then injected into mice. part of the digested samples was used to ...200111390071
diversity of ectoparasites in sheep flocks in são paulo, brazil.the occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. sheep production used to be a predominantly family activity in the state of são paulo (brazil), but it began to become a commercial activity in the past decade. thus, information about the ectoparasites existing in sheep flocks has become necessary. the present data were obtained by means of questionnaires sent to all sheep breeders belonging to the 'associação paulista de criadores de ovinos' (aspaco; ...200011020945
effect of management practices on screw-worm among sheep in são paulo state, brazil.the screw-worm cochliomyia hominivorax (coquerel), whose larvae develop in wild and domestic animals as well as in man, is one of the major causes of myiasis in brazil. sheep raising is expanding in the state of são paulo, where information about the infestation of screw-worm is necessary for appropriate control measures. the present data were obtained from questionnaires sent to all sheep breeders belonging to associacão paulista de criadores de ovinos--aspaco (são paulo state association of sh ...19989719843
host specificity of sheep and cattle nematodes in são paulo state, brazil.the trial was carried out to investigate parasite host specificity and to analyse the dynamics of infection with nematodes parasitizing sheep and cattle raised together or separately in são paulo state, brazil, and, also to clarify doubts about the systematics of species of the genus haemonchus on the basis of cytological and morphological studies. ten steers and 32 ewes were randomly assigned to three paddocks (p), as follows: p1, 5 steers; p2, 5 steers and 16 ewes; and p3, 16 ewes. the animals ...19979477496
a prospective clinical study of the mononucleosis syndrome in a developing country.a prospective aetiologic analysis made of 70 children and adults with the clinical and haematologic features of infectious mononucleosis (im) in sao paulo, brazil, revealed 65.7% due to ebv (31 heterophile antibody positive (ha+), 15 ha negative (ha-) cases), 8.6% due to t. gondii and 4.3% due to cmv. one case was related to viral hepatitis and one to drug hypersensitivity. the other 18.6% were of unknown cause. in the 46 im cases positive tests were found for ebv/igm in 93.5%, for heterophile b ...19806259069
epidemiology of fasciola hepatica infection in the paraíba river valley, são paulo, brasil.the study revealed that although lymnaea columella and stenophysa marmorata were found in the same habitat, only l. columella harbored intramolluscan stages of f. hepatica. the population density of l. columella cyclically decreased between september and february, and increased from march to september. the drought that occurred between june and november 1981 dramatically reduced the number of snails collected. larger snails were collected in march-july and november-december, while the smaller sn ...19863564331
virulence factors in escherichia coli strains isolated from pigs in the ribeirao preto region, state of sao paulo, brazil.three-hundred faecal swabs were obtained from pigs with diarrhoea in farms located in different areas of the ribeirao preto region in the state of sao paulo. one-hundred escherichia coli strains were isolated and tested for production of thermolabile (tl) and thermostable (stra and stb) enterotoxins, and for the presence of colonization factors f4, f5 and f6. the strains were also tested for sensitivity to 14 antibiotics and chemotherapeutic agents. twenty-four escherichia coli strains produced ...19911775690
species of coccidia occurring in lambs in são paulo state, brazil.the purpose of this experiment was to characterize the species of eimeria affecting lambs and their infection pattern. faecal samples were collected from each animal at 14-day intervals, beginning when lambs were 2 weeks old and ending when they were 32 weeks old. the oocysts were counted and identified as e. intricata, e. parva, e. pallida, e. crandallis, e. bakuensis, e. weybridgensis, e. ahsata and e. ovinoidalis. the highest oocyst counts were observed when the lambs were 4-8 weeks old.19921502781
epidemiology of sheep myiases in são paulo state, brazil.a survey of diptera species causing cutaneous myiases on sheep in botucatu, são paulo, brazil was made to determine seasonal incidence, predilection sites and the factors predisposing to infestation. sheep were checked daily for myiases for one year. at two week intervals larvae from wounds were collected for identification. only larvae of dermatobia hominis and cochliomyia hominivorax were found. myiases due to c. hominivorax were observed during the whole year with high incidence from january ...19921306916
dermatophilosis in sheep from são paulo (brazil). 1977859515
contribution to the epidemiology of paracoccidioidomycosis and histoplasmosis in the state of sáo paulo, brazil. paracoccidioidin and histoplasmin intradermic tests in domestic animals. 1978694719
[occurrence of gaigeria pachyscelis railliet & henry, 1910 (nematoda: ancylostomidae) parasite of ovines in the state of são paulo, brasil (author's transl)]. 1977564685
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