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experimental elisa for diagnosis of ovine dicrocoeliosis and application in a field survey.dicrocoeliosis is a parasitic disease caused by trematodes belonging to the genus dicrocoelium. it usually produces mild symptoms, and for this reason, dicrocoeliosis often remains undetected. its diagnosis is mostly based on postmortem examination of the liver or on coprological assays for in vivo diagnosis. however, the latter method has scant sensitivity and because of the long pre-patency of dicrocoelium spp. only permits late diagnosis. in the present study, an enzyme-linked immunosorbent a ...200919107521
seroprevalence, clinical incidence, and molecular and epidemiological characterisation of small ruminant lentivirus in the indigenous passirian goat in northern italy.eight dairy flocks, comprising a total of 323 indigenous passirian goats from northern italy, were examined to determine the seroprevalence and clinical incidence of small ruminant lentivirus (srlv) infections and to identify the srlv subtypes. the seroprevalence was 81.5% (55-95%). the clinical incidence was 2.5% (0-8.3%) and was apparently low due to the practice of culling clinically affected animals. phylogenetic analysis of eight pcr fragments (one sample from each flock) revealed that all ...200818584116
prevalence of antibodies to leptospira serovars in sheep and goats in alto adige-south tyrol.serum samples from 313 sheep and 95 goats were collected during november 1993 in 26 localities in alto adige-south tyrol and tested by microscopic agglutination test for antibodies to 28 serovars of the genus leptospira. at the time of blood collection all the animals appeared healthy with no clinical sign suggestive of leptospirosis. the observed seroprevalence in sheep was 6.1%, whereas the seropositivity rate for goat serum samples was 2.1%. the highest serological prevalence in sheep was rec ...200010829576
antibodies to borrelia burgdorferi in sheep and goats. alto adige-south tyrol, italy.a serologic survey for antibodies to borrelia burgdorferi was conducted on sheep and goat serum samples collected in alto adige-south tyrol, italy, in 1990. sera were tested by indirect immune fluorescence assay (iifa) and microbial adherence immobilization assay (maia). iifa and/or maia anti-b. burgdorferi antibodies were detected in 14.1% of the 269 sheep and 36.8% of the 133 goats examined. iifa and maia were both positive in 4 out of 38 positive sheep sera (10.5%) and 21 out of 49 positive g ...19968722314
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