Publications

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interspecific interactions in naturally acquired nematode communities from sheep abomasum in relation to age of host and season in four areas of león (spain).the abomasa of naturally infected ewes (407) and lambs (190) from four areas of león were collected weekly over 2 years in the abattoir of león (north-west spain). the major species and morphs found were teladorsagia circumcincta and trichostrongylus axei and to a lesser extent marshallagia marshalli and teladorsagia trifurcata. the putative interactions between species were assessed by euclidian distances based on the results of multivariate analyses. the estimation of interaction was either ba ...19921639568
dynamics of the elimination of dicrocoelium dendriticum (trematoda, digenea) eggs in the faeces of lambs and ewes in the porma basin (león, nw spain).the elimination of dicrocoelium dendriticum eggs in sheep faeces from 4 localities in the upper and middle porma river basin was recorded at monthly intervals between march 1986 and march 1987. the faecal material was taken directly from the rectum of the animals (10% of the flock in each village). the sedimentation technique and mcmaster egg counting chambers were used to analyze the faecal samples. of the 995 samples examined, 63.6% contained d. dendriticum eggs and the number of eggs per gram ...19911952698
epizootiology of eimeria ahsata coccidiosis in león (spain).the epizootiology of ovine coccidiosis caused by eimeria ahsata was studied in four flocks in the province of león (spain), between january 1978 and december 1979. the intensity of infection was found to be 25.5 +/- 1.7% and was similar in winter and spring. eimeria ahsata was the most common species in 83.1% of the 1620 samples examined. it was also found in 86.2% of the animals examined. only 0.3% of the positive samples contained oocysts of a single species, and samples containing four specie ...19883369071
ticks of the province of salamanca (central/nw spain). prevalence and parasitization intensity in dogs and domestic ungulates.by sporadic examination of 492 wild animals and regular weekly checks of dogs (179), cattle (288) and sheep and goats (141), 13 ixodides were identified in the province of salamanca. some of these were only detected on the northern (i. ricinus) or southern (h. lusitanicum and b. annulatus) slopes of the mountains to the south of the province. except for occasional introductions, h. lusitanicum and b. annulatus are not found on the castillian plateau. in wild animals, 12 species were found of whi ...19863706991
seroprevalence of fasciola hepatica infection in sheep in northwestern spain.to estimate the prevalence of fasciola hepatica infection in sheep in the león province (northwestern spain), we conducted a survey between october 1992 and may 1993. a total of 767 samples of feces and serum were collected from sheep over 1 year of age belonging to 152 flocks randomly selected from the 4 natural regions of león province. samples were analyzed by a standard coprological sedimentation method and an indirect enzyme-linked immunosorbent assay (elisa) using excretory-secretory produ ...19957731921
[seroprevalence of the infections caused by borrelia burgdorferi and rickettsia conorii in humans and dogs in primary health care of san andreas del rabanedo (leon, spain)].positive results in infections of borrelia burgdorferi and rickettsia conorii in human and canine population is studied in order to understand the situation of both in humans, and at the same time discover the importance of the dog as an indicator of these agents among those in the health area of san andrés del rabanedo, león.19979546860
characterization of adult dicrocoelium dendriticum by isoelectric focusing.water soluble extracts of 3131 adult specimens of dicrocoelium dendriticum from cattle, sheep and goats, mainly from león province, were analysed by isoelectric focusing in thin-layer polyacrylamide gels. activity of the following enzymes was studied: lactate dehydrogenase (ldh, ec 1.1.1.27), glucose phosphate isomerase (gpi, ec 5.3.1.9), phosphoglucomutase (pgm, ec 2.7.5.1), acid phosphatase (acp, ec 3.1.3.2), alpha-glycerophosphate dehydrogenase (alpha-gpdh, ec 1.1.1.8), hydroxybutyrate dehydr ...19989687590
soil type as a putative risk factor of ovine and caprine paratuberculosis seropositivity in spain.relationships between soil type and ovine and caprine paratuberculosis in the avila region (central spain) were evaluated using data from a cross-sectional study of the most-important diseases of small ruminants in this spanish region between 1996 and 1997. questionnaire data from 61 herds (38 ovine and 23 caprine) and 1451 serum samples (1041 ovine and 410 caprine) were used. herd paratuberculosis (herds were scored as positive to paratuberculosis if any of the serum samples was positive in an ...200010665950
isolation of actinobacillus seminis from the genital tract of rams in spain.actinobacillus seminis isolates were cultured from the semen (two isolates) and the left testis (one isolate) of two one-year-old rams in león, spain. one animal showed lesions of chronic unilateral orchitis and epididymitis while the other appeared to suffer a subclinical infection and only a moderate number of pleomorphic rods and inflammatory cells were present in its semen. the isolates were biochemically similar to the a. seminis type strain nctc 10851 and two other european a. seminis isol ...200010684692
risk factors for brucellosis seroprevalence of sheep and goat flocks in spain.risk factors for ovine and caprine brucellosis in the avila region (center of spain) were evaluated using data from a cross-sectional study of the most important diseases of small ruminants in this spanish region between 1996 and 1997. questionnaire data from 56 herds (35 ovine and 21 caprine) were used. sixteen (29%) flocks (3 caprine and 13 ovine) were brucellosis-seropositive. overall, 0.7% of sheep and 0.1% of goats were seropositive. eleven risk factors were studied at the group level by lo ...200010760400
[outbreak of q fever and seroprevalence in a rural population from soria province].the aim of this study was to describe an outbreak of acute fever in a rural town from soria (spain) in the spring of 1998 and to know the prevalence of igg antibodies to coxiella burnetii in this population.200111333569
evaluation of official eradication-campaigns data for investigating small-ruminant brucellosis in the province of león, spain.brucellosis official eradication-campaigns data were used to study the epidemiology of the small-ruminant brucellosis infection in the province of león (northwest spain). data from three consecutive campaigns (1997-1999) were retrieved from flock sheets from controlled flocks and from other official statistics. in general, flock information was scarce, with a small percentage of contradictory data and other deficiencies that might have affected the campaign results. nevertheless, unconditional m ...200111535281
contributions to and review of dicrocoeliosis, with special reference to the intermediate hosts of dicrocoelium dendriticum.an epidemiological study on dicrocoeliosis caused by dicrocoelium dendriticum was carried out on sheep, molluscs and ants in the mountains of león province (nw spain) between 1987-1991. the results concerning the intermediate hosts and a review of some aspects of dicrocoeliosis are summarized. mollusc collection for the helminthological study was random throughout the study area at fortnightly intervals. twenty-nine gastropoda species were identified. d. dendriticum infection was only detected i ...200111769295
epidemiological study of rickettsia typhi infection in two provinces of the north of spain: analysis of sera from the general population and sheep.data relating to rickettsia typhi infection in spain are scarce. a serological survey of 383 serum samples (184 males, 199 females) from the general population and 120 sera from sheep were studied by immunofluorescence assay (ifa). the human serum samples were collected from the general population of palencia and burgos provinces, and sheep serum samples were collected from palencia province. of the 383 human serum samples studied, 29 were positive for antibodies against r. typhi (7.5%) no stati ...200516011432
field and experimental studies on dicrocoelium dendriticum and dicrocoeliasis in northern spain.the transmission, control and the relationship between dicrocoelium dendriticum and its definitive (sheep and cattle) and intermediate (molluscs and ants) hosts under natural and experimental conditions are described. eleven species of molluscs and four of ants were found infected with larval d. dendriticum in león province, north-west spain. infected ants were observed between april and november and in tetania at 7.5-26.9 degrees c. the highest shedding of eggs by sheep and cattle was detected ...200516336711
diagnosis of the nervous form of maedi-visna infection with a high frequency in sheep in castilla y leon, spain.between 1997 and march 2004, the nervous form, or visna, of maedi-visna infection was diagnosed in 71 of 1631 sheep (4.35 per cent) examined in the castilla y león region of spain, of which 634 had shown nervous signs. the presence of the virus was confirmed by immunohistochemistry and in some cases by pcr on frozen-thawed or paraffin-embedded tissue samples. the main clinical signs were hindleg ataxia and paresis, but blindness or nystagmus were also observed. thirty-three of the affected sheep ...200616489160
detection, occurrence, and characterization of escherichia coli o157:h7 from raw ewe's milk in spain.a study was carried out in the castilla y león region of spain to investigate the presence of escherichia coli o157:h7 in raw ewe's milk samples collected from several cheese factories during 1 year. all specimens were examined for e. coli o157:h7 by selective enrichment at 41.5 +/- 1.0 degrees c, after both 6 and 22 h of incubation, and then immunomagnetically separated and plated on cefixime-potassium tellurite-sorbitol macconkey agar. no growth was obtained in the enrichment broth after a 6-h ...200616629039
echinococcus granulosus infection in spain.cystic echinococcosis (ce) caused by the cestode echinococcus granulosus is an endemic disease in spain. although specific control programmes initiated in the 1980s have led to marked reductions in ce infection rates in spain, the disease still remains an important human and animal health problem in many regions of the country. human incidence and livestock (including sheep, cattle, pigs and horses) prevalence data were gathered from national epidemiological surveillance information systems and ...200818331519
[transhumance, the wool trade, and credit: the compania de ganaderos de las provincias de soria y burgos, 1781-1800]. 200119655460
development and evaluation of an antibody elisa for sarcoptic mange in sheep and a comparison with the skin-scraping method.in this work an indirect elisa for detecting serum-specific igg antibodies in sheep was developed using a crude saline extract from sarcoptes scabiei var. ovis mites and then the repeatability of the elisa outcomes was estimated. subsequently, its diagnostic accuracy was evaluated by receiver operating characteristics (roc) analysis using a sample collected from the entire sheep population of western castile and leon region in spain, and then compared with that of the skin-scraping method. the r ...201020594604
cross-sectional sero-epidemiological survey of sarcoptic mange in sheep of western castile and leon, spain.the aim of the present study was to determine the prevalence of sarcoptes scabiei-infection of ovine livestock in three provinces (leon, zamora and salamanca) in the western part of the castile and leon region in spain, and to determine the association between different variables and seropositivity. a total of 3730 sheep sera from 373 flocks (10 sera from each flock) collected from may to september over the course of the years 2006 and 2007 were individually analysed by an indirect antibody elis ...201020691485
structure and performance of awassi and assaf dairy sheep farms in northwestern spain.data of 69 dairy sheep farms (70% assaf and 30% awassi crossbred), located in the spanish autonomous community of castilla y león and grouped for receiving technical advice, were used to study their structure and performance. farm surface was 55.4ha, on average. approximately 25% of the farms did not have cultivation land, and the other 75% had, on average, 73ha (from which 67% were devoted to forage). farms used 2.1 annual work units (familiar, 90%), 493 ewes, and yielded 147,000 l/yr of milk. ...201121257045
prevalence of ectoparasitic arthropods on wild animals and cattle in the las merindades area (burgos, spain).this paper reports the prevalence of ectoparasitic arthropods in sampled groups of wild (n = 128; 16 species) and domestic (n = 69; 3 species) animals in the las merindades area of the province of burgos, spain. the study revealed that wild animals were more infested and with a wider variety of ectoparasites than domestic animals. the parasitic prevalence was 67% for wild animals and 48% for livestock. in this way, 39% of animals were infected by ticks. ixodes ricinus and ixodes hexagonus were t ...201121894267
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