antimicrobial susceptibility of corynebacterium pseudotuberculosis isolated from lesions of caseous lymphadenitis in sheep in hokkaido, japan. 19911830812
seroepidemiological survey of corynebacterium pseudotuberculosis infection in sheep in japan using enzyme-linked immunosorbent assay and immunodiffusion.sera from 1186 apparently healthy sheep in hokkaido were examined by enzyme-linked immunosorbent assay (elisa) and immunodiffusion (id) for the presence of antibodies against corynebacterium pseudotuberculosis. elisa-positives were 466 (39.3%) while id-positives were 330 (27.8%). spread of caseous lymphadenitis in sheep in hokkaido was thus clarified. although id was less sensitive than elisa in detecting the antibodies against c. pseudotuberculosis it did not give any non-specific reaction. fro ...19892514314
isolation and serological survey of chlamydia psittaci in sheep in hokkaido. 19883172598
seroepizootiological survey on bluetongue virus infection in cattle in japan.bovine sera collected in various parts of japan were subjected to seroepizootiological tests with bluetongue virus type 1 (btv1), type 12 (btv12), and type 20 (btv20). all these viruses have been widely disseminated among cattle in the southern part of japan in 1974. relatively high incidences of neutralizing (nt) antibody against the three viruses were shown among cattle in the kyushu district, including okinawa prefecture, or the southern part of japan, but extremely low or incidences in hokka ...19826312332
survey of natural scrapie in japan: analysis of rflp types of the prp gene and detection of prpsc mainly in suffolk sheep.we examined the brains, spleens and/or lymph nodes of 197 mainly suffolk sheep collected from hokkaido, and the tohoku, kanto and chubu districts to detect prpsc and thus to estimate scrapie contamination in japan. sixteen sheep in hokkaido and 2 sheep in other districts that were introduced from hokkaido were positive for prpsc. by comparison of the frequencies of the restriction fragment length polymorphism (rflp) types of these 18 scrapie sheep with 128 healthy sheep, we confirmed the associa ...19947999881
detection of sheep-associated malignant catarrhal fever virus antibodies by complement fixation tests.some serological diagnosis methods and examinations for detection of antibodies to sheep-associated malignant catarrhal fever (mcf) infection were investigated. the wildebeest-associated mcf virus strain wc11 propagated on fetal bovine thyroid cell cultures was used as an antigen. antibodies were detected by complement fixation (cf) tests in cattle pathologically diagnosed as having sheep-associated mcf, as well as in cattle experimentally infected with mcf virus strain wc11. however, immunodiff ...19968645750
high prevalence of borna disease virus infection in healthy sheep in japan.previous seroepidemiological and molecular epidemiological studies of borna disease virus (bdv) showed considerably high rates of infection in horses, cattle, cats, and humans in hokkaido, japan. here, we further demonstrate high rates of specific antibodies to bdv and bdv rna in peripheral blood mononuclear cells (pbmcs) from healthy sheep bred on the same island. the bdv prevalences by immunoblotting and/or reverse transcriptase pcr were 0% (0 of 19) in newborns (<1 month old), 51.7% (15 of 29 ...19979144374
japanese scrapie cases.worldwide attention has been given to scrapie, because bovine spongiform encephalopathy (bse) could be experimentally transmitted to sheep. this ovine form of bse was clinically identical to scrapie. in japanese scrapie cases, a majority of the diseased sheep were from suffolk, while 8 cases were from corriedale. it is very likely that sheep-to-sheep transmission of scrapie has taken place in obihiro, hokkaido. normal prion protein may play a role in the morphoregulatory signaling pathway, which ...200010871915
epidemiological survey of border disease virus among sheep from northern districts of japan.the first epidemiological survey of border disease virus (bdv) was undertaken in small ruminants in japan. ovine sera, collected from the northern prefectures of hokkaido, aomori and iwate, were examined for the presence of antibodies against bdv using the neutralization peroxidase-linked antibody test. twenty-nine (17.6%) of one hundred and sixty-five samples were seropositive for bdv. results were specific, excluding cross-reactions with bovine viral diarrhea virus (bvdv). only one sample (0.6 ...201121778667
surveillance of jaagsiekte sheep retrovirus in sheep in hokkaido, the northern island of japan.surveillance of jaagsiekte sheep retrovirus (jsrv) infection was performed by polymerase chain reaction (pcr) of blood dna samples collected from 40 sheep and goats in 10 different flocks in hokkaido, the northern island of japan. no exogenous (oncogenic) jsrv sequence was detected by the pcr in these samples, while the ovine endogenous retrovirus sequence was successfully amplified in all samples. our paper is the first demonstration of jsrv surveillance in japan, and shows no evidence of oncog ...201121712639
seroepidemiological survey of sheep flocks from northern japan for mycoplasma ovipneumoniae and mycoplasma agalactiae.sheep flocks from hokkaido, iwate and aomori, three northern prefectures of japan, were screened for antibodies to mycoplasma ovipneumoniae and mycoplasma agalactiae by elisa. sixty four animals out of 246 (26%) were seropositive to m. ovipneumoniae, with positive results obtained from all three prefectures. none of the sera tested were serologically positive to m. agalactiae.201121830171
A Clinical Case of Severe Anemia in a Sheep Coinfected with Mycoplasma ovis and 'Candidatus Mycoplasma haemovis' in Hokkaido, Japan.A 2-year-old East Friesian sheep imported from Australia exhibited severe anemia after contagious pustular dermatitis in Hokkaido, Japan. Hemoplasma infection was confirmed in blood smears. Both Mycoplasma ovis and 'Candidatus Mycoplasma haemovis' were detected by PCR and sequence analyses. In the epidemiological analysis, dual pathogens were detected in 6 of 12 (50.0%) sheep imported from Australia with the infected ewe at the same time, 1 of 5 (20.0%) sheep introduced from a domestic farm in H ...201121869571
Epidemiological survey for visna-maedi among sheep in northern prefectures of Japan.Ovine sera collected from the northern Prefectures of Hokkaido, Iwate and Aomori in Japan, were examined for the presence of antibodies against visna-maedi virus using the agar gel immunodiffusion test and enzyme-linked immunosorbent assays. Three animals (1.12%), out of 267 samples tested, were found to be seropositive to the visna-maedi antigens in both tests. Levels of infection were found in flocks from Hokkaido and Iwate Prefectures, but not in the Aomori Prefecture. Nucleic acid detection ...201122194226
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