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genetic variability and population structure in beta-lactoglobulin, calpastain and calpain loci in iranian kurdi sheep.the genotypes for beta-lactoglobulin (blg) and calpastatin (cast) were determined by polymerase chain reaction (pcr) and restriction enzyme digestion and genotyped for calpain (capn) by pcr-sscp method in an iranian breed sheep, kurdi. blood samples were collected from 100 pure bred kurdi sheep from kurdi breeding station located in shirvan, mashhad. the extraction of genomic dna was based on guanidin thiocyanate-silica gel method. after pcr reaction, amplicons were digested with restriction enz ...200719070051
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